蛞蝓多糖体外抗乙型肝炎病毒作用研究

目的:研究蛞蝓多糖体外抗乙型肝炎病毒的作用。方法:以不同浓度的蜗牛多糖和拉米夫定与HepG2.2.15细胞混合培养,通过MTT法检测药物的细胞毒性,9d后用ELISA法检测细胞培养液中的HBsAg和HBeAg分泌水平,用实时荧光定量PCR技术检测培养液中的HBV-DNA含量。结果:蛞蝓多糖在1mg·mL^-1浓度以下对细胞无毒性。蛞蝓多糖在所稀释的各浓度下,对HBsAg、HBeAg均有抑制作用,最大抑制率分别为56.2%和58.1%;HBsAg、HBeAg的治疗指数分别大于19.98%、22.78%。蛞蝓多糖可抑制HBV-DNA的复制(P<0.05)。结论:蛞蝓多糖在体外具有显著抑制HBV的作用。

Studies on Anti-HBV Effect in vitro of Limax Polysaccharide

Objective:To study the in-vitro inhibitory of limax polysaccharide on hepatitis B virus. Methods:The cell toxicity of limax polysaccharide was determined with MTT method,and the effect of limax polysaccharide on HBsAg and HBeAg secreted by HepG2.2.15 cells was evaluated with ELISA method on the ninth day of incubation. real-time PCR was used for determining secrected HBV-DNA in the culture medium. Results:limax polysaccharide had no obvious cell toxicity at the concentration less than 1 mg·mL^-1 and depressed duplication on HBV-DNA. Limax polysaccharide had obvious restrain effect on HBsAg and HBeAg with maximum inhibition ratio addig up to 56.2%and 58.1%. The therapeutic index of limax polysaccharide was 19.98% for HBsAg and 22.78% for HBeAg. There was certain inhibition on replication of HBV-DNA(P<0.05). Conclusion:Limax polysaccharid has no obvious immediate cell toxicity on the HepG 2.2.15 cell and can inhibit duplication of hepatitis B virus in vitro.