| 时间分辨荧光分析技术的研究进展及应用 |
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| 引用本文： | 周伟玲,赵启仁. 时间分辨荧光分析技术的研究进展及应用[J]. 国际放射医学核医学杂志, 2006, 30(2): 103-106 |
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| 作者姓名： | 周伟玲 赵启仁 |
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| 作者单位： | 300192 天津, 中国医学科学院、中国协和医科大学放射医学研究所实验核医学室 |
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| 基金项目： | 天津市自然科学基金资助项目(043610211) |
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| 摘 要： | 时间分辨荧光分析(TRFA)技术是一种以镧系元素螫合物为标记物,测量其发射荧光的超灵敏的无放射性污染的标记分析技术,在生物分析中得到了广泛的应用。在近些年,研究发展了酶放大时间分辨荧光分析、时间分辨猝灭分析和分子信标等分析系统,其灵敏度更高,满足了不同的需求,使TRFA技术在临床诊断和科学研究中得到了更广泛的应用。
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| 关 键 词： | 荧光免疫测定 稀土元素 荧光共振能量转移 |
| 文章编号： | 1673-4114(2006)02-0103-04 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005-06-13 |
The development and application of time-resolved fluorescence assay |
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| ZHOU Wei-ling,ZHAO Qi-ren. The development and application of time-resolved fluorescence assay[J]. International Journal of Radiation Medicine and Nuclear Medicine, 2006, 30(2): 103-106 |
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| Authors: | ZHOU Wei-ling ZHAO Qi-ren |
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| Affiliation: | Department of Experimental Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China |
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| Abstract: | Time-resolved fluorescence assay is a new nonisotopic and ultrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have been gotten a broader application in clinical diagnosis and science research. |
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| Keywords: | Fluoroimmuno assay Lanthanide series elements Fluorescence resonance energy transfer |
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