肝素化胶原/壳聚糖多孔支架的制备及其血管化的研究

本研究旨在构建一种能快速血管化的人工真皮替代物。用冻干法制备了胶原／壳聚糖多孔支架，并对其进行肝素化，观察此支架的结构特征、亲水性、体外降解性和组织相容性，同时将血管生成素引入到此支架，对复合有血管生成素的肝素化支架的体内血管化进行了初步研究。结果表明，肝素化胶原／壳聚糖多孔支架具有合适的三维多孔结构、良好的吸水性和较理想的酶解稳定性，体内实验表明，此支架具有良好的组织相容性，血管生成素可加快支架的血管化。

Study on Fabrication of Heparinized Collagen/chitosan Porous Scaffold and its Angiogenesis in vivo

To develop an artificial dermal substitute capable of receiving rapid angiogenesis.Collagen/chitosan porous scaffold was fabricated by freeze-drying method,and heparinized using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide(EDC) and N-hydroxysuccinimide(NHS).The morphology,hydrophilicity,in vitro biodegradation and tissue compatibility of the scaffold were investigated.After combined with angiogenin,the in vivo angiogenesis of the scaffold was studied as well.The results showed that with a suitable three-dimensional porous structure,the heparinized collagen/chitosan porous scaffold possessed good hydrophilicity and stability for collagenase degradation.In vivo study also demonstrated that the heparinized scaffold had good tissue compatibility,and its angiogenesis could be enhanced by angiogenin.