| 大豆异黄酮干预对乳腺癌中ERK1、ERK2和c-Fos表达的影响及意义 |
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| 引用本文： | 侯建峰,冯笑山,杨晓鹤,米建强,刑鲁奇,王公平,张广平,高社干,马志坤,郝曙光,段瑞华,陈强,茹艺.大豆异黄酮干预对乳腺癌中ERK1、ERK2和c-Fos表达的影响及意义[J].肿瘤,2010,30(9). |
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| 作者姓名： | 侯建峰 冯笑山 杨晓鹤 米建强 刑鲁奇 王公平 张广平 高社干 马志坤 郝曙光 段瑞华 陈强 茹艺 |
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| 作者单位： | 1. 河南科技大学第一附属医院肿瘤科,洛阳,471003
2. 美国俄克拉荷马大学病理中心,俄克拉荷马,73104 |
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| 基金项目： | 河南省医学科技攻关项目 |
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| 摘 要： | 目的:观察大豆异黄酮(soybean isoflavones)干预MMTV-erbB-2转基因小鼠,对乳腺癌组织中细胞外信号调节激酶1/2(extracellular signal regulated kinase 1/2,ERK1/2)和c-Fos蛋白表达的影响及意义.方法:选择鼠龄3周的健康MMTV-erbB-2转基因雌性小鼠100只,随机分为4 组(早期干预组、晚期干预组、全程干预组和对照组),观察各组小鼠乳腺癌的发病率和潜伏期,采用免疫组织化学法检测各组小鼠乳腺癌组织及正常乳腺组织中ERK1、ERK2和c-Fos蛋白的表达并进行统计学分析.结果:早期干预组、晚期干预组、全程干预组和对照组雌性MMTV-erbB-2转基因小鼠乳腺癌的发病率分别为48%、28%、20%和92%;与对照组比较,早期干预组、晚期干预组和全程干预组发病率均降低(P<0.01);全程干预组发病率低于早期干预组(P<0.05),晚期干预组与早期干预组和全程干预组比较,发病率差异均无统计学意义;各组小鼠乳腺癌潜伏期分别为(47.67±9.25)、(47.29±14.13)、(51.8±6.69)和(49.91±8.07)周,各组间差异均无统计学意义.各组小鼠的肿瘤直径比较差异无统计学意义(P＞0.05);ERK1、ERK2和c-Fos在乳腺癌组织中的阳性表达率分别为71.6%、68.7%和61.2%,在小鼠正常乳腺组织中阳性表达率分别为27.7%、19.1%和12.8%,差异有统计学意义(P<0.01);与对照组相比,早期干预组、晚期干预组和全程干预组乳腺癌组织ERK1、ERK2和c-Fos阳性表达均有所降低,但差异无统计学意义(P＞0.05).结论:大豆异黄酮对MMTV-erbB-2转基因雌鼠乳腺癌的发生具有一定的预防作用,丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)信号转导通路在乳腺癌的发生、发展过程中可能起着重要作用.
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| 关 键 词： | 乳腺肿瘤 疾病模型 动物 异黄酮类 |
Interference of the expression of ERK1, ERK2, and c-Fos in breast cancer by soybean isoflavones and its significance |
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| HOU Jian-feng,FENG Xiao-shan,YANG Xiao-he,MI Jian-qiang,XING Lu-qi,WANG Gong-ping,ZHANG Guang-ping,GAO She-gan,MA Zhi-kun,HAO Shu-guang,DUAN Rui-hua,CHEN Qiang,RU Yi.Interference of the expression of ERK1, ERK2, and c-Fos in breast cancer by soybean isoflavones and its significance[J].Tumor,2010,30(9). |
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| Authors: | HOU Jian-feng FENG Xiao-shan YANG Xiao-he MI Jian-qiang XING Lu-qi WANG Gong-ping ZHANG Guang-ping GAO She-gan MA Zhi-kun HAO Shu-guang DUAN Rui-hua CHEN Qiang RU Yi |
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| Institution: | HOU Jian-feng1,FENG Xiao-shan1,YANG Xiao-he2,MI Jian-qiang1,XING Lu-qi1,WANG Gong-ping1,ZHANG Guang-ping1,GAO She-gan1,MA Zhi-kun2,HAO Shu-guang1,DUAN Rui-hua1,CHEN Qiang1,RU Yi1(1.Department of Oncology,First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,China,2.Pathology Center,University of Oklahoma,Oklahoma 73104,USA) |
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| Abstract: | Objective:To investigate the effects of soybean isoflavones on the expressions of extracellular signal regulated kinase 1/2(ERK1/2) and c-Fos in breast cancer of MMTV-erbB-2 transgenic mice and its significance.Methods:One hundred 3-week old healthy MMTV-erbB-2 transgenic female mice were divided into four groups: early intervention group,advanced intervention group,whole-course intervention group and control group.The incidence rate and the latent period of breast cancer were observed.The expression of ERK... |
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| Keywords: | ERK1 ERK2 c-Fos |
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