| A Comparative Study on Several Models of Experimental Renal Calcium Oxalate Stones Formation in Rats |
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| 作者姓名： | 刘继红 曹正国 张朝辉 周四维 叶章群 |
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| 作者单位： | Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
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| 摘 要： | In order to compare the effects of several experimental renal calcium oxalate stones formation models in rats and to find a simple and convenient model with significant effect of calcium oxalate crystals deposition in the kidney, several rat models of renal calcium oxalate stones formation were induced by some crystal-inducing drugs (CID) including ethylene glycol (EG), ammonium chloride (AC), vitamin D3 1α(OH)VitD3, alfacalcidol], calcium gluconate, ammonium oxalate, gen-tamicin sulfate, L-hydroxyproline. The rats were fed with drugs given singly or unitedly. At the end of experiment, 24-h urines were collected and the serum creatinine (Cr), blood urea nitrogen (BUN), the extents of calcium oxalate crystal deposition in the renal tissue, urinary calcium and oxalate ex-cretion were measured. The serum Cr levels in the stone-forming groups were significantly higher than those in the control group except for the group EG L-hydroxyproline, group calcium gluconate and group oxalate. Blood BUN concentration was significantly higher in rats fed with CID than that in control group except for group EG L-hydroxyproline and group ammonium oxalate plus calcium gluconate. In the group of rats administered with EG plus Vitamin D3, the deposition of calcium ox-alate crystal in the renal tissue and urinary calcium excretion were significantly greater than other model groups. The effect of the model induced by EG plus AC was similar to that in the group in-duced by EG plus Vitamin D3. EG plus Vitamin D3 or EG plus AC could stably and significantly in-duced the rat model of renal calcium oxalate stones formation.
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| 关 键 词： | 实验研究 钙离子 结石 草酸钙 |
| 收稿时间： | 2005-10-25 |
A comparative study on several models of experimental renal calcium oxalate stones formation in rats |
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| Liu Jihong,Cao Zhengguo,Zhang Zhaohui,Zhou Siwei,Ye Zhangqun.A Comparative Study on Several Models of Experimental Renal Calcium Oxalate Stones Formation in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(1):83-87. |
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| Authors: | Liu Jihong Cao Zhengguo Zhang Zhaohui Zhou Siwei Ye Zhangqun |
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| Institution: | Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China |
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| Abstract: | Summary In order to compare the effects of several experimental renal calcium oxalate stones formation models in rats and to find
a simple and convenient model with significant effect of calcium oxalate crystals deposition in the kidney, several rat models
of renal calcium oxalate stones formation were induced by some crystal-inducing drugs (CID) including ethylene glycol (EG),
ammonium chloride (AC), vitamin D31α(OH)VitD3, alfacalcidol], calcium gluconate, ammonium oxalate, gentamicin sulfate, L-hydroxyproline. The rats were fed with drugs given
singly or unitedly. At the end of experiment, 24-h urines were collected and the serum creatinine (Cr), blood urea nitrogen
(BUN), the extents of calcium oxalate crystal deposition in the renal tissue, urinary calcium and oxalate excretion were measured.
The serum Cr levels in the stone-forming groups were significantly higher than those in the control group except for the group
EG+L-hydroxyproline, group calcium gluconate and group oxalate. Blood BUN concentration was significantly higher in rats fed
with CID than that in control group except for group EG+L-hydroxyproline and group ammonium oxalate plus calcium gluconate.
In the group of rats administered with EG plus Vitamin D3, the deposition of calcium oxalate crystal in the renal tissue and urinary calcium excretion were significantly greater than
other model groups. The effect of the model induced by EG plus AC was similar to that in the group induced by EG plus Vitamin
D3. EG plus Vitamin D3 or EG plus AC could stably and significantly induced the rat model of renal calcium oxalate stones formation.
LIU Jihong, male, born in 1963, Professor, M.D., Ph.D. |
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| Keywords: | calcium oxalate rat model crystal-inducing drug |
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