DNA转染淋巴细胞表达宫颈癌细胞膜抗原

目的：证明抗宫颈癌单克隆抗体（mＡb）ＡＵ１４－１识别的宫颈癌抗原决定簇是肿瘤相关基因表达的产物。方法：将宫颈癌（Ｕ１４）细胞ＤＮＡ转染原代培养正常淋巴细胞；分别用细胞和夹心酶联免疫吸附试验（ＥＬＩＳＡ）检测ＤＮＡ转染细胞膜表面抗原。结果转染细胞如同Ｕ１４细胞一样对ＡＵ１４－１染色呈阳性反应；western印迹法分析表明，转染 细胞膜表面可溶性抗原呈现４条与Ｕ１４细胞一致的分子量为２００００、４６０００、６

DNA- transfected lymphocytes expressing uterine cervical cancer cell membrane antigen

AIM: To demonstrate that uterine cervical cancer antigenic determinant recognized by mAb (monoclonal antibody) to uterine cervical cancer (AU14-1) is a product expressed by tumor-related genes. METHODS:The primary culture of normal lymphocytes was transfected with DNA preparation derived from uterine cervical cancer (U14) cells. The membrane surface antigens of DNA-transfected cells were detected with cellular ELISA and sandwich ELISA respectively.RESULTS:It was indicated that transfected lymphocytes showed the same positive reaction against AU14-1 as U14 cells did. By using Western blot analysis, it was found that the transfectant membrane surface soluble antigens showed four protein bands with molecular weights of 20 000, 46 000, 61 000 and 65 000, similar to that of U14 cells. CONCLUSION: The membrane surface antigen epitopes on uterine cervical cancer cells were low molecular weight glycoprotein coded by tumor-related genes.