山莨菪碱对肝脏缺血再灌注损伤的保护作用

目的 探讨山莨菪碱对肝脏缺血再灌注损伤的保护作用。方法 将雄性Wistar大鼠制成肝脏缺血再灌注模型，随机分为正常对照组、缺血再灌注组、生理盐水组和山莨菪碱组，观察肝脏缺血60min再灌注1、3、6、12及24h后血浆和／或肝组织中内皮素－1（ET－1）、透明质酸（HA）、丙氨酸转氨酶（ALT）、丙二醛（MDA）和再灌注1h后肝细胞内游离Ca^2 (Ca^2 ]i)、ATP含量变化以及肝组织病理学改变。结果 肝脏缺血再灌注后血浆和／或肝细胞中ET－1、HA、ALT、MDA和肝细胞内Ca^2 ]u含量均显著升高，而肝组织中ATP含量明显降低；肝脏缺血再灌注前应用山莨菪碱2.0mg/kg者，血浆HA和肝细胞内Ca^2 ]i含量明显降低，肝组织中MDA也有不同程度的降低，而肝组织中ATP含量明显升高，同时肝酶的漏出减少，肝组织病理学损害明显减轻。结论 山莨菪碱对肝脏缺血再灌注损伤具有保护作用。

The protective mechanism of anisodamine on liver ischemia-reperfusion injury in rats

Objective To study the protective mechanism of anisodamine on liver ischemia-reperfusion injury in rats.Methods One hundreds and sixty male Wistar rats were randomly divided into the normal control group (n=10), ischemia-reperfusion group (n=50), physiologic saline group (n=50)and anisodamine group (n=50). The animals were killed at 1st,3rd, 6th,12th,24th h after ischemia induced for 60 min and followed by reperfusion. Plasma and(or) liver endothelin-1 (ET-1), hyaluronic acid (HA), glutamic-pyruvic transaminase enzyme (ALT) and malondialdehyde (MDA) were measured. Liver intracellular i and adenosine triphosphate (ATP) after being reperfused for 1 h were measured. Meanwhile, the histopathologic hepatic alterations were also observed.Results Plasma ET-1, HA, ALT and liver ET-1, MDA, intracellular i concentrations were markedly increased and liver ATP markedly decreased after liver ischemia-reperfusion. It was found that an intravenous injection of anisodamine (2.0 mg/kg) before ischemia-reperfusion could significantly decrease the levels of HA, ALT, MDA, intracellular i and increase ATP concentrations in plasma and(or) liver. Meanwhile, the histopathologic hepatic alterations were significantly lessened.Conclusions The anisodamine have protective effects on liver ischemia-reperfusion injury.