庚型病毒性肝炎17例的临床和病原学分析

目的:对南京地区庚型病毒性肝炎的临床和病原学特点进行分析。方法:用反转录聚合酶链反应(RT-PCR)法检测血清HGV-RNA。从274例病毒性肝炎患者中检测出17例庚型病毒性肝炎,观察其临床表现及血清病原学标志,并分析庚型肝炎病毒(HGV)部分核酸序列。结果与结论:17例患者男性成年人多见,全年散发,经输血感染为重要传播途径。HGV可以单独感染,也可重叠(混合)其它肝炎病毒感染。少数为急性肝炎,多数为慢性肝炎或肝硬化,尤其在重叠(混合)感染。单纯HGV感染者症状轻,多隐匿发病,肝功能损害较轻。重叠(混合)感染者多有慢性肝炎的症状,与HBV重叠(混合)感染时有形成重型肝炎的趋势。核酸序列分析表明HGV南京株部分核苷酸序列与HGV美国株HGU 44402、HGU 45966、HGU 36380及HGV河北株在对应位置的核苷酸同源性从87.27%~93.94%,可能HGV有不同的基因型。

Clinical and Seroetioligical Analysis of Viral Hepatitis G

Aims: To study the clinical features of viral hepatitis G and HGV-RNA type in Nanjing area. Methods: Seventeen patients with hepatitis G were collected from in-patients with HGV-RNA positive (RT-PCR assay). The markers of hepatitis A to G were detected in all patients and HGV-RNA sequence were analysed in the patients. Results: Eight of 17 patients had blood transusion history. There were 8 cases of patients with pure HGV infection and the other 9 cases had superinfection with HBV and/or HCV. Eight patients with pure HGV infection patients had no or slight symptoms, 2 patients showed chronic sererious hepatitis in 3 cases with HGV HBV superinfection and 7 patiens showed chronic hepatitis or cirrhosis. The HGV-RNA sequence analysis showed that the HGV Nanjing strain had 82. 27% -93.94% sequence homology with HGV American strains and Hebei strain. Conclusions: Hepatitis G may result from blood transfusion and also result from other infectious ways. There are pure HGV infection and superinfection with HBV and/or HCV in hepatitis G. Patients with pure HGV infection show no or slight symptoms. And HGV infection based on hepatitis B may result in chronic serious hepatitis. The distribution of HGV genotypes is probably region-specific.