云南白药对大鼠血小板聚集及膜糖蛋白表达的影响

目的研究大鼠服用云南白药后,血小板在ADP及花生四烯酸诱导下聚集率的变化,以及血小板糖蛋白CD61及CD62P在静息及ADP诱导条件下在膜表面表达的变化.方法大鼠ig给药3d后,取血,用血小板聚集仪测定血小板的聚集率以及用流式细胞仪测定CD61和CD62P的表达.结果无论在ADP或花生四烯酸诱导下,云南白药组的血小板聚集率都显著高于对照组(P<0.01);在静息条件下,CD61及CD62P在膜表面的表达虽有少量增加,但与对照组相比没有显著差异(P>0.05),但在ADP刺激条件下,给药组CD61及CD62P表达比对照组都有显著增加(P<0.01).结论在诱导条件下,云南白药可以促进血小板聚集及血小板膜上CD61及CD62P的表达,但不会增加静息血小板表面CD41及CD62P的表达,不会形成促血栓倾向.

Effects of Yunnang Baoyao on the expression of platelet membrane glycoproteins

OBJECTIVE To study the alterations of aggregation rate of platelets induced by ADP or arachidonic acid and to study the change of membrane glycoproteins of resting platelets or platelets activated by ADP after administration of Yunnang Baoyao. METHOD After SD rats were administrated with Yunnang Baoyao for three days, the platelet aggregation rate was detected by platelet aggregation analyzer, and the expression of CD41 and CD62P on platelets membrane was detected by flow cytometry. RESULTS The platelet aggregation rate of groups administrated with Yunnang Baoyao was much higher than control group (P<0.01). If the platelets were activated by ADP, the expression of CD61 and CD62P on the platelets membrane in Yunnang Baoyao group was increased significantly compared with the control group. However, there was no significant change of these glycoproteins in resting platelets among groups (P>0.05).CONCLUSION Yunnang Baoyao, under stimulating condition, can increase the reaction activity of platelet and the expression of CD61,CD62P on the membrane. Howerer, Yunnang Baoyao will not increase its glycoproteins expression on platelet membrane under normal condition.