肾苏Ⅱ对局灶节段性肾小球硬化病理形态学的影响

目的:以局灶节段性肾小球硬化为研究对象,观察辛通畅络、益气和血中药复方肾苏Ⅱ对病理形态学的影响,探讨中医药防治疑难疾病的有效途径。方法:采用阿霉素注射法诱发大鼠局灶节段性肾小球硬化型阿霉素肾病动物模型,将实验动物随机分为对照组、模型组、西药组和中药组。于治疗后第12周末运用光、电镜,观察各组大鼠肾脏病理形态学变化,半定量分析肾小球硬化积分、肾小球PAS染色阳性区面积及肾小球系膜基质相对面积。结果:病理形态观察显示,肾苏Ⅱ可减轻肾脏病理损害,抑制肾小球硬化及细胞外基质积聚,且优于贝那普利。结论:肾苏Ⅱ对阿霉素注射法诱发大鼠局灶节段性肾小球硬化型阿霉素肾病模型具有保护作用。通过改善病理形态学变化,达到阻抑或减缓局灶节段性肾小球硬化进展的效果。

The Iinterventions of Shensu II About the Pathological Modalities of Focal Segemental Glomerulosclerosis

Objective：To make focal segmental glomerulosclerosis（FSGS）as the research aim,observe and discuss the effects of the renal function tests and pathological modalities by the traditional Chinese drug complex prescrription-ShensuⅡ which is to dredge vessels by xintong and to complement qixue.Find the effectual modes from traditional Chinese medicine to prevention and cure the diseases which is difficult to make a diagnosis and give treatment.Methods：Rat focal segmental glomerulosclerosis animal model is induced by injecting doxo -rubicin hydrochoride. The experimental animals are randomly divided into groups,the comparison group,the disease model group,the western medicine group and the Chinese medicine group. At the end of 12th week of treatment phase,observe pathological modality changes by optical microscope,electron microscope,analyse cirrhosis integral,PAS coloration area and extracellular matrix relative area.Results：ShensuⅡ can improve renal pathological modalities and check cirrhosis and accumulation of ECM.The curative effects of ShensuⅡ are better than western medicine in improving renal pathological modalities and checking cirrhosis and accumulation of ECM.Conclusion：ShensuⅡ can protect focal segmental glomerulosclerosis doxorubicin nephropathy model which is induced by injecting doxorubicin hydrochoride.Check and mitigate FSGS progress by improving pathological modalities.