鹅绒委陵菜多糖抗肿瘤作用研究

目的探讨鹅绒委陵菜多糖（PAP）的抗肿瘤作用。方法采用MTT法研究PAP对体外培养人肝癌细胞SMMC-7721的增殖抑制作用；并将s180荷瘤小鼠随机分组，灌胃给予50，100，200mg·kg-1剂量的PAP，研究PAP对荷瘤小鼠的抑瘤率和免疫功能的影响。结果体外实验结果显示PAP对SMMC-7721细胞的体外增殖有显著抑制作用，400mg·L-1PAP组抑制率最高，为43．57％；PAP对人肝癌SMMC-7721细胞的抑制作用呈剂量依赖性，拟合曲线呈线性相关。动物实验结果显示低、中、高3个剂量的PAP对S180荷瘤小鼠的抑瘤率分别为46．41％，52．29％和59．48％，其中环磷酰胺和低剂量的多糖组合抑瘤率与中、高剂量的多糖抑瘤率相当，并显著高于单用环磷酰胺组的抑瘤率；同时PAP能够显著提高T-淋巴细胞和巨噬细胞吞噬率。结论PAP具有抗肿瘤活性和增强免疫的功能。

Anticancer Effects of Polysaccharide from Potentilla anserine L.

OBJECTIVE To study the antitumor effects and immunological function of polysaccharide from Potentilla anserine L.(PAP) in vivo and in vitro. METHODS The SMMC-7721 cells were treated with different concentration of PAP. The effects of PAP on proliferation of human hepatoma SMMC-7721 cell were examined by MTT assay in vitro, and the relative results were fitted with mathematical model. The S180-bearing mice were intragastrically administered with 50, 100, 200 mg·kg^-1 PAP, respectively. The inhibition rate of S180 tumor and immunological function of PAP were measured in vivo. RESULTS The effects on proliferation of SMMC-7721 cell were correlated with the concentration of PAP in vitro. The highest inhibition was 43.57% at the dose of 400 mg·L^-1 PAP. The effects and PAP concentration were correlated and regressed. The inhibiting tumor rates were 46.41%, 52.29% and 59.48% in different groups treated with 50, 100 and 200 mg·kg^-1 in vivo, respectively. The T-lymphocytes and macrophage phagocytic index increased significantly after the effects of PAP in S180-bearing mice. CONCLUSION The compound PAP had significant activities of antitumor and immunological function.