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Borna disease virus RNA in peripheral blood mononuclear cells obtained from healthy dairy cattle |
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| Authors: | Katsuro Hagiwara Takaaki Nakaya Yurie Nakamura Sayumi Asahi Hirokazu Takahashi Chiaki Ishihara K Ikuta |
| Institution: | (1) Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 060 Japan Tel./Fax: 81-11-707-6837; e-mail: kiimm.hokudai.ac.jp, JP;(2) Department of Animal Model, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan, JP |
| Abstract: | We examined natural infection of Borna disease virus (BDV) in 74 healthy dairy cattle in Japan. The seroprevalence determined using recombinant BDV p24 protein was 20.3%. The prevalence of BDV RNA in peripheral blood mononuclear cells (PBMCs) determined by polymerase chain reaction for p24 was 10.8%. Cell fraction of the PBMCs revealed the presence of BDV RNA in the T cell-enriched fraction. Thus, BDV infection is wide-spread even among healthy cattle. Received: 12 August 1996 |
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