| 异丙酚干预星形胶质细胞形态学变化及水通道蛋白-4的表达 |
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| 引用本文： | 陈寒鉴,潘彩飞,郭鹏,郑跃英,祝胜美. 异丙酚干预星形胶质细胞形态学变化及水通道蛋白-4的表达[J]. 中国神经再生研究, 2011, 6(5): 383-387 |
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| 作者姓名： | 陈寒鉴 潘彩飞 郭鹏 郑跃英 祝胜美 |
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| 作者单位： | Department of Anesthesiology, First Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China,浙江大学医学院附属第一医院,绍兴县中心医院,浙江大学医学院附属第一医院,浙江大学医学院附属第一医院 |
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| 摘 要： | Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown. In the present study, astrocytes were cultured in vitro and exposed to ammonium chloride (NH4Cl), followed by propofol, protein kinase C agonist, or antagonist, respectively. Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis. Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4Cl-induced astrocyte swelling and inhibited increased aquaporin-4 expression. Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4Cl; protein kinase C pathway activation is potentially involved.
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| 关 键 词： | ammonium chloride aquaporin-4 astrocytes propofol protein kinase C |
Effects of propofol on ammonium chloride-exposed astrocyte morphology and aquaporin-4 expression |
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| Chen Hanjian,Pan Caifei,Guo Peng,Zheng Yueying and Zhu Shengmei. Effects of propofol on ammonium chloride-exposed astrocyte morphology and aquaporin-4 expression[J]. Neural Regeneration Research, 2011, 6(5): 383-387 |
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| Authors: | Chen Hanjian Pan Caifei Guo Peng Zheng Yueying Zhu Shengmei |
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| Affiliation: | Department of Anesthesiology, First Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China,Department of Anesthesiology, First Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China,Department of Anesthesiology, Shaoxing Central Hospital, Shaoxing 312030, Zhejiang Province, China,Department of Anesthesiology, First Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China,Department of Anesthesiology, First Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310003, Zhejiang Province, China |
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| Abstract: | Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown. In the present study, astrocytes were cultured in vitro and exposed to ammonium chloride (NH4Cl), followed by propofol, protein kinase C agonist, or antagonist, respectively. Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis. Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4Cl-induced astrocyte swelling and inhibited increased aquaporin-4 expression. Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4Cl; protein kinase C pathway activation is potentially involved. |
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| Keywords: | ammonium chloride aquaporin-4 astrocytes propofol protein kinase C |
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