| 细胞膜色谱技术在多肽类成分筛选的研究进展 |
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| 作者姓名： | 孙铁锋 王平 丁相龙 丁立钧 姜其宝 王金国 |
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| 作者单位： | 1.山东省中医药研究院，山东 济南 250014;1.山东省中医药研究院，山东 济南 250014；2.天津大学精密测试技术及仪器国家重点实验室，天津 300072;3.青岛市黄岛区第二中医医院，山东 青岛 266400;4.日照市中医医院，山东 日照 276800;5.山东中医药大学，山东 济南 250355 |
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| 基金项目： | 山东省中医药科技项目（No.2020Q023）；山东省自然科学基金（No.ZR2020MH386）；国家重点研发计划政府间国际科技创新合作项目（No.2019YFE0117800）；中央引导地方科技发展专项基金（No.YDZX2021117）；山东省重点研发计划（科技示范工程）（No.2021SFGC1205） |
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| 摘 要： | 近年来，随着色谱技术的不断发展，细胞膜色谱在多肽类活性物质的研究中得到了广泛的应用。本文在总结国内外相关文献的基础上，介绍了细胞膜色谱技术的原理和特点、细胞膜色谱技术的分类以及细胞色谱技术在多肽类活性成分的研究进展。总结并提出了细胞膜色谱检测多肽的优点、目前存在的问题，展望了前景。
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| 关 键 词： | 细胞膜色谱 多肽类成分 筛选 |
Research progress of cell membrane chromatography in screening active components of polypeptides |
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| Authors: | SUN Tiefeng WANG Ping DING Xianglong DING Lijun JIANG Qibao WANG Jinguo |
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| Institution: | 1.Shandong Academic of Chinese Medicine,Jinan 250014,China;2.State Key Laboratory of Precision Testing Technology and Instruments, Tianjin University,Tianjin 300072,China;3.Huangdao District Second Hospital of Traditional Chinese Medicine, Qingdao 266400,China;4.Rizhao Hospital of Traditional Chinese Medicine,Rizhao 262300,China;5.Shandong University of Traditional Chinese Medicine,Jinan 250355,China |
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| Abstract: | In recent years, with the continuous development of chromatography technology, cell membrane chromatography has been widely used in the study of polypeptide active substances. On the basis of summarizing the relevant literature at home and abroad, this paper introduces the principle and characteristics of cell membrane chromatography, the classification of cell membrane chromatography and the research progress of cell chromatography in polypeptide active components. The advantages and existing problems of polypeptide detection by cell membrane chromatography were summarized and put forward, and the prospect was prospected. |
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| Keywords: | Cell membrane chromatography Polypeptide components Screen |
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