Biochemical characterization of an aphthovirus type 0(1) strain campos attenuated for cattle by serial passages in chicken embryos |
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Authors: | J M Parisi P Costa Giomi P Grigera P Augé de Mello I E Bergmann J L La Torre E A Scodeller |
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Affiliation: | 1. Centeo de Virologia Animal (CEVAN), Serrano 661 (1414) Capital Federal, Argentina,;2. Pan American Foot-and-Mouth Disease Center, P.O. Box 589, CEP 10001, Rio de Janeiro, Brazil |
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Abstract: | The biochemical properties of a virulent and an attenuated strain of foot-and-mouth disease virus (FMDV) Type 0(1) Campos (0(1)C) were compared in order to establish differences that could account for their altered biological functions. The avirulent strain (0(1)C-O/E) was derived from the virulent strain 0(1)C by serial passages in chicken embryos. Analysis of the RNase T1-generated oligonucleotides of the viral RNA through one- and two-dimensional (2D) gel electrophoresis (fingerprints) revealed a few changes in the genome structure of the 0(1)C-O/E strain compared to the wild type strain. In addition there was a significant decrease in the length of the poly(C) rich tract of the 0(1)C-O/E RNA. All virion structural proteins, except VP4, their precursors, and the viral RNA polymerase (p56a) show charge differences. In addition a significant decrease in the apparent molecular weight of polypeptide p100 (primary translational product from the 3' end region of the genome) of the attenuated strain was observed. |
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Keywords: | Author to whom requests for reprints should be addressed. |
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