饮水氟暴露对成年男性性激素结合球蛋白及睾酮水平的影响

目的探讨饮水氟暴露对成年男性性激素结合球蛋白(SHBG)及睾酮水平的影响。方法依据水氟检测结果,在河南省通许县随机选择3个村庄作为调查点,分别为高氟村、改水村和对照村;整群抽取调查区本地生长的18～50岁成年男性,收集空腹静脉血和晨尿。应用氟离子选择电极法测定饮用水和尿中氟的含量;用酶联免疫吸附法(ELISA)测定其血清中SHBG水平,化学发光法测定血清睾酮的含量。结果血清SHBG浓度分别为对照组47.85nmol/L,改水组31.37nmol/L,高氟组24.52nmol/L,3组比较差异均有显著性(P<0.05)。血清睾酮水平分别为对照组3.69ng/ml,改水组4.61ng/ml,高氟组4.83ng/ml,高氟与对照组比较,差异有显著性(P<0.05)。高氟组血清SHBG水平与睾酮呈正相关关系(r=0.230,P=0.049),而在改水组和对照组中未观察到相关关系。结论长期氟暴露可影响成年男性血清SHBG水平和睾酮水平,并可能存在剂量-效应关系。

Influence of water fluoride exposure on sex hormone binding globulin and testosterone in adult male

Objective To explore the influence of water fluoride exposure on sex hormone binding globulin(SHBG) and testosterone in adult male.Methods Cross-sectional study was conducted in three villages of Tongxu county including high fluoride group(HFG),defluoridation project group(DFPG) and control group(CG) based on the fluoride concentration in drinking water.Adult male who were born and raised in the village and aged 18-50 years old were recruited using cluster sampling.Fasting blood and morning urine samples were collected.The fluoride levels in drinking water and urine were detected by fluoride-ion selective electrode method.Serum SHBG level was determined using enzyme-linked immunosorbent assay(ELISA).The chemical luminescence immune analysis method was used to detect serum testosterone content.Results Serum SHBG level was 47.85nmol/L in CG,31.37nmol/L in DFPG and 24.52nmol/L in HFG respectively.There were significant difference among of three groups(P<0.05).Serum testosterone level was 3.69ng/ml in CG,4.61ng/ml in DFPG and 4.83ng/ml in HFG respectively.Serum testosterone level in HFG was significantly higher than that in CG(P<0.05).Serum SHBG level in HFG has positive correlation with serum testosterone(r=0.230,P=0.049),which has not been observed in DFPG and CG.Conclusions Long-time fluorine exposure may affect serum SHBG and testosterone level in adult male.