| RP-HPLC法测定维药白花丹中白花丹醌的含量 |
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| 引用本文： | 孙宇,;卿德刚,;张娟,;倪慧,;贾晓光,;李超颖. RP-HPLC法测定维药白花丹中白花丹醌的含量[J]. 西北药学杂志, 2014, 0(2): 127-129 |
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| 作者姓名： | 孙宇, 卿德刚, 张娟, 倪慧, 贾晓光, 李超颖 |
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| 作者单位： | [1]新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002; [2]新疆大学生命科学与技术学院,乌鲁木齐830046 |
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| 基金项目： | 国家药品（维药）标准提高项目 |
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| 摘 要： | 目的建立维吾尔药材白花丹中白花丹醌含量测定方法。方法采用反相高效液相色谱法,色谱柱为Waters XTerra RP C18(250mm×4.6mm,5μm);流动相为甲醇-水溶液;体积流量为1.0mL·min-1;检测波长为270nm;柱温25℃。结果在上述条件下,白花丹醌进样量在0.028 60.457 6μg范围内呈良好的线性关系,加样回收率为100.4%,RSD为1.2%。结论该方法简便、准确、灵敏度高、重复性好,能有效测定白花丹药材中白花丹醌的含量。
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| 关 键 词： | 白花丹 白花丹醌 RP-HPLC 维药 |
Determination of plumbagin in Uygur medicine Plumbago zey lanica L .by RP-HPLC |
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| Affiliation: | SUN Yu, QING Degang, ZHANG Juan, NI Hui, JIA Xiaoguang, LI Chaoying (1. Xinjiang Institute of Chinese Materia Med ica and Ethical Materia Medica, Urumqi 830002, Chinal 2. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China) |
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| Abstract: | Objective To establish a method for the determination of plumbagin in Plumbago zeylanica L ..Methods Plumbagin was determined by RP-HPLC on a Waters XTerra RP C18 （250 mm ＆#215; 4 .6 mm ,5μm） column with methanol-water as the mobile phase . The flow rate was 1 .0 mL ＆#183; min-1 ,the detection wavelength was 270 nm ,and the column temperature was 25 ℃ .Results The cal-ibration curve of plumbagin was linear within the range of 0 .028 6-0 .457 6 μg and the average recovery was 100 .4% （RSD=1.2% ） .Conclusion The method is simple ,accurate ,sensitive and reproducible .It could be used for the determination of plumba-gin in Plumbago zeylanica L .. |
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| Keywords: | RP-HPLC plumbagin RP-HPLC Uygur medicine |
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