| HPLC法测定和血明目片中黄芩苷的含量 |
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| 引用本文： | 田园,;王海亮,;解笑瑜,;王嗣岑. HPLC法测定和血明目片中黄芩苷的含量[J]. 西北药学杂志, 2014, 0(4): 361-363 |
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| 作者姓名： | 田园, 王海亮, 解笑瑜, 王嗣岑 |
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| 作者单位： | [1]西安交通大学药学院,西安710061; [2]西安碑林药业股份有限公司,西安710048 |
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| 摘 要： | 目的建立和血明目片中黄芩苷的HPLC含量测定方法。方法采用反相高效液相色谱法。C18色谱柱;流动相为甲醇-磷酸-水溶液(47∶0.2∶53);检测波长为280nm;流速为1.0mL·min-1;柱温30℃;峰面积外标法定量。结果黄芩苷在0.026 20.393 6mg·mL-1范围内呈良好的线性关系,平均回收率96.3%,RSD为1.6%(n=6)。结论该方法简便,结果准确可靠,可作为该制剂的质量控制方法。
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| 关 键 词： | 和血明目片 黄芩苷 高效液相色谱法 含量测定 |
Determination of baicalin in Hexuemingmu Tablets by HPLC |
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| Affiliation: | TIAN Yuan, WANG Hailiang, XIE Xiaoyu, WANG Sicen(1. School of Pharmacy, Xi＇ an Jiaotong University, Xi＇ an 710061, China; 2. Xi＇an Beilin Pharmaceutical Limited Company, Xi＇an 710048, China) |
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| Abstract: | Objective To establish an HPLC method for the determination of baicalin in Hexuemingmu Tablets.Methods The HPLC method was carried out on a C18column using methanol-phosphoric acid-water（47∶0.2∶53）as mobile phase.The detection wavelength was 280nm,the flow rate was 1.0mL·min^-1,and the temperature of column was 30℃.The baicalin in Hexuemingmu Tablets was determined by HPLC-peak area external standard method.Results The calibration curve for baicalin was linear in the range of 0.026 2-0.393 6mg·mL^-1.The average recovery was 96.3% with RSD of 1.6%（n=6）.Conclusion The method is simple,precise and reliable for determining the content of baicalin in Hexuemingmu Tablets. |
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| Keywords: | Hexuemingmu Tablets baicalin HPLC content determination |
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