香青兰总黄酮对支气管哮喘大鼠肺功能的影响

目的观察香青兰总黄酮对支气管哮喘大鼠肺功能及肺组织III型胶原的影响。方法sD大鼠60只随机分为正常对照组、模型组、地塞米松组和香青兰总黄酮低、中、高剂量组。除正常对照组外，其余各组采用卵白蛋白（OVA）致敏和激发建立大鼠哮喘模型。模型建立后，正常对照组和模型组给予双蒸水灌胃，各给药组分别给予香青兰总黄酮和地塞米松灌胃，1次／d，连续30d。末次给药后，采用美国Buxco公司的整体体积描记系统，检测香青兰总黄酮对支气管哮喘大鼠呼吸频率（F）、每分钟通气量（MVb）、吸气流量峰值（PIFb）及呼气流量峰值（PEFb）及增强的呼吸间歇（Penh）的影响，ELISA法检测肺组织III型胶原含量变化。结果香青兰总黄酮180、360mg／kg可降低支气管哮喘大鼠F和Penh（P〈0.05，P〈0．01），升高MVb、PIFb和PEFb（P〈0．05），降低肺组织III型胶原含量（P〈0．05，P〈0．01）。结论香青兰总黄酮可改善支气管哮喘大鼠肺功能，降低气道反应性，改善哮喘气道重塑。

Effect of Moldavica Total Flavone on Pulmonary Function in Ovalbumin-induced Bronchial Asthmatic Rats

Objective To investigate the effect of moldavica total flavone on pulmonary function and collagen type III in lung tissue of ovalbumin-induced bronchial asthmatic rats. Methods A total of 60 SD rats were randomly divided into control group, model group, dexamethasone group, and moldavica total flavone low, medium and high dose group. Rat asthma model was established by ovalbumin challenge methods except the control group. The control and model group were intragastrically given distilled water, medicine groups were intragastrically given moldavica total flavone and dexamethasone for 30 d, once per day. After the last administration, the pulmonary function and airway responsiveness including breathing frequency （F）, minute ventilation （MVb）, peak inspiratory ＇flow （PIFb）, peak expiratory flow （PEFb） and enhanced pause （Penh） were measured by using non-invasive measurement system （Penh system, Buxco, USA）. The collagen type III in lung tissue were detected by ELISA. Results The moldavica total flavone at 180 and 360 mg/kg decreased F and Penh （P〈0.05, P〈0.01）, increased MVb, PIFb and PEFb （P〈0.05）, and decreased collagen type III in lung tissue （P〈0.05, P〈0.01）. Conclusion Moldavica total flavone can relieve the airway hyperresponsiveness and remodeling, improve respiratory function of ovalbumin- induced bronchial asthmatic rats.