消痰散结方对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定的影响

目的探讨消痰散结方对MKN-45人胃癌裸鼠原位移植瘤微卫星不稳定的作用机制。方法采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源，采用0B胶黏合法建立人胃癌裸鼠原位移植瘤模型。20只模型裸鼠分为模型组和中药组。中药组予消痰散结方干预6周后，测瘤质量，计算抑瘤率并检测5个微卫星不稳定位点（D17S250、D2S123、D5S346、Bat-25和Bat-26）。结果中药组的瘤质量明显低于模型组（P〈0．01），其抑瘤率为40．84％。模型组的肿瘤组织中能扩增到5个微卫星位点，其中微卫星位点D2S123、D5S346出现某些延长和缩短。使用消痰散结方干预6周后，微卫星位点D2S123、D5S346的变异趋于稳定。结论消痰散结方能抑制MKN-45人胃癌裸鼠原位移植瘤的微卫星不稳定。

Anticancer Effects of Xiaotansanjie Recipe on Microsatellite Instability in MKN-45 Human Gastric Cancer Orthotopic Transplantation

Objective To explore the mechanism of Xiaotansanjie recipe on MKN-45 human gastric cancer orthotopic transplantation tumor with microsateUite instability. Methods MKN-45 human gastric cancer cell line was used to establish subcutaneous tumor model in nude mice as an experimental model which passed three generations of tumor source. Then OB glue was used to establish human gastric cancer orthotopic xenograft model. Twenty model nude were divided into model group and Xiaotansanjie recipe group （treated with Xiaotansanjie recipe）. After 6 weeks of treatment intervention, tumor weight was measured, inhibitory rate was calculated and microsatellite instability （D17S250, D2S123, D5S346, Bat-25 and Bat-26） was detected. Results The tumor weight in Xiaotansanjie recipe group was significantly lower than that in model group （P〈0.01）. The tumor-inhibition rate was 40.84%. Five microsatellite markers （D17S250, D2S123, D5S346, Bat-25 and Bat-26） were amplified in the tumor tissue of model group, and microsatellite markers D2S123, D5S346 appeared some prolong and reduction. MicrosateUite markers （D2S123, D5S346） mutation was stabilized after 6 weeks treatment of Xiaotansanjie recipe. Conclusion Xiaotansanjie recipe can inhibit the MKN-45 human gastric cancer orthotopic transplantation tumor by down-regulating the microsatellite instability.