益肺清化颗粒对Lewis肺癌小鼠血管内皮细胞生长因子及其受体的影响

目的观察益肺清化颗粒对LewiS肺癌荷瘤小鼠瘤体中的血管内皮细胞生长因子（VEGF）及其受体（KDR）含量的影响，以期阐明其抑瘤的作用机制。方法80只C57BL／6近交系小鼠右腋皮下接种LewiS瘤细胞悬液0．2mL（细胞浓度为1×10^7个／mL），随机分为空白组、对照组、益肺清化颗粒低剂量组、益肺清化颗粒中剂量组、益肺清化颗粒高剂量组、吉非替尼组、吉非替尼＋益肺清化颗粒中剂量组、环磷酰胺（CTX）组。每组10只。实验第2日开始给药，除CTX组第3日及第7日给药2次外，其余各组均给药14d。实验第15日处死小鼠，取瘤，以蛋白质印迹法（WesternB10t）检测瘤组织VEGF及其受体KDR的表达。结果益肺清化颗粒低、中剂量组VEGF表达低于对照组（P〈0．01），吉非替尼组VEGF表达低于对照组（P〈0．05），益肺清化颗粒高剂量组、吉非替尼＋益肺清化颗粒中剂量组、CTX组VEGF表达低于对照组（P〈0．001）；益肺清化颗粒低、中、高剂量组，吉非替尼组及CTX组KDR表达与对照组比较均有所降低（P〈0．05）。结论益肺清化颗粒可降低VEGF及其受体KDR的表达，进而发挥抑瘤作用。

Influence of Yifeiqinghua Granules on Expression of Vascular Endothelial Growth Factors and Its Receptor KDR in Lewis Lung Cancer Mice

Objective To clarify the mechanism of Yifeiqinghua granules on tumor angiogenesis and tumor inhibition by observing its influence on the expression of vascular endothelial growth factor （VEGF） and its receptor KDR in Lewis lung cancer mice. Methods Eighty C57BL/6 inbred mice subcutaneously planted with Lewis tumor cell suspension 0.2 mL were randomly divided into 8 groups： blank group, control group, low-, medium- and high-dose of Yifeiqinghua granules groups, Gefitinib group, Gefitinib plus medium-dose of Yifeiqinghua granules group and CTX group, 10 mice in each group. Drugs were administrated from the second day. CTX group was administered on third day and seventh day, other groups were administered for 14 days. The mice were killed to take the tumor on the 15th days. VEGF and KDR expression in tumor tissue was measured by Western Blot. Results Compared with the blank group, the expression of VEGF was significantly decreased in the low- and medium-dose of Yifeiqinghua granules groups （P〈0.01）. Gefitinib group＇s VEGF value was lower than the blank group （P〈0.05）. Compared with the blank group, the expression of VEGF was significantly decreased in the high-dose of Yifeiqinghua granular group, Gefitinib plus medium-dose of Yifeiqinghua granules group and CTX group （P 〈0.001）. Compared with the blank group, the expression of KDR was significantly decreased in the low-, medium- and high-dose of Yifeiqinghua granules groups, Gefitinib group and CTX group （P 〈0.05）. Conclusions The possible mechanism of Yffeiqinghua granules in treating non-small cell lung cancer is reducing the expression of VEGF and KDR, thus play an inhibitory effect.