维拉帕米对血栓形成及血清一氧化氮水平的影响

目的:探讨维拉帕米(Verapamil)对于血栓形成以及血小板聚集率的影响及其可能的机制.方法:大鼠体内分别给予不同剂量的维拉帕米(5,10,20 mg/kg)、赖氨匹林(4.5 mg/kg)以及溶剂(生理盐水2 ml).利用大鼠颈总动脉-颈外静脉血流旁路法观察维拉帕米对血栓形成的影响,并与赖氨匹林比较;同时观察维拉帕米对于血小板聚集率的影响;提取血清,通过硝酸还原酶法测定一氧化氮(NO)代谢产物,以反映NO的水平.结果:维拉帕米可以呈浓度依赖性的降低血小板的聚集率,明显降低血栓的形成,结论:维拉帕米可能通过提高NO的水平来改善血小板的功能.

Effect of verapamil on the thrombogenesis and nitric oxide level in the serum of rats

Objective:To study the effect of verapamil on platelet aggregation,thrombogenesis and the potential mechanism.Methods:The SD rats were injected the different concentration of verapamil,Aspirin-DL-Lysine and the saline solution for 7 days(i.p),respectively.After the administration,the platelet aggregation was measured by aggregometry,the thrombogenesis was tested by the pathway of arteria carotis communis-external jugular vein,and the concentration of NO was evaluated with the kit of nitrate reductase.Results:Verapamil exhibited a dose-dependent inhibitory effect on platelet aggregation and thrombus formation in rats.Conclusion:Verapamil imporoving the function of platelet was through elevating the NO level.