自配试剂免疫透射比浊比率法测定血清CIC

目的:寻求一种全自动仪测循环免疫复合物(CIC)的新方法,以取代传统方法繁琐的手工劳动和略显粗糙的定量结果。方法:自配PEG和BBS试剂,以康宁560全自动生化仪结合AU560全自动生化仪设置程序最佳参数,免疫透射比浊终点法多点定标IPEG和BBS,比率法计算CIC值,以百分回收试验和重复性试验确定其准确度和精密度,以对照试验与传统PEG沉淀法作对比分析。结果:仪测CIC最佳参数设置为:血清:试剂=1:8.λ_1340nm,λ_2450 nm,内源性读数时间20 Sec,总体反应时间288 See,总反应体积为180μl,37℃。本方法与传统PEG沉淀法的回收率、日内和日间变异系数(CV%)分别为97%～110%、3.5%、4.3%和90%～122%、6.8%、9.7%。与传统PEG沉淀法相比,准确性差异无显著性(P>0.05),两者具有较好的相关性(r=0.852),精密度则比手工方法高。结论:自配试剂上机检测CIC,其操作简便易行,且定量相对准确,重现性好,线性范围宽,完全可以取代繁琐的传统操作。

Serum CIC Determination By Immunological Transmission Turbidimetry Ratio with Self-mix Reagents

Objective To look for a new full-automatic instrumental method for Circulating Immune Complexes (CIC) de- termination,to replace the tedious handicraft labor of traditional method and gain more accurate results.Methods Try to mix BBS and PEG reagents self,establish the best procedure parameters with Kangning 560 combine with AU560,immunological transmission turbidimetry 4 points calibration the PEG and BBS,compute the CIC value with ratio method,then judge its accu- racy and degree of precision by percentage recovery ratio study and repeatability study,make contrast experiment to compare with traditional PEG precipitation method.Results The best procedure parameters of CIC instrumental method were:Serum: Reagents=1:8,λ1340 nm,λ2450 nm,endogenous read time 20 Sec,total reaction time 288 Sec,total reaction volume 180 ul, 37℃.Percentage recovery ratio,inside day and daytime percentage variation coeffiicient(CV%) of this method and traditional PEG precipitation method respectively for 97%～110%,3.5%,4.3% and 90%～122%,6.8%,9.7%.Compared with tra- ditional PEG precipitation method,it has no difference in accuracy(P>0.05),the two have good correlation(r=0.852),but degree of precision is higher than handicraft method.Conclusion Serum CIC determination by immunological transmission tur- bidimetry ratio with Self-mix reagent,its operation is simple and convenient,also it shows quantitative measurement relatively accurate,good repeatability,wide linear,it may replace nitty-gritty traditional hand-operation completely.