针刺小肠经不同腧穴拮抗庆大霉素耳毒性的特异性研究

目的 :通过针刺小肠经不同腧穴观察小肠经腧穴与耳的相关性 ,比较其主治特异性。方法 :将豚鼠随机分为正常组、模型对照组、针刺“听宫”组、针刺“肩贞”组、针刺“后溪”组 ,每组 12只。除正常组外 ,其余 4组以庆大霉素肌注 (每日 80mg/kg ,连续 2 0d) ,造成庆大霉素耳毒性模型 ,造模同时针刺组每日分别针刺“听宫”“肩贞”“后溪”穴 ,每次 3 0min ,每日 1次 ,连续 2 0d。观察各组琥珀酸脱氢酶 (SDH)染色耳蜗铺片、听毛细胞缺失数、热休克蛋白活性表达及透射电镜下听毛细胞的超微结构变化。结果 :针刺“听宫”组耳蜗铺片、听毛细胞缺失数、听毛细胞超微结构情况均明显好于模型对照组 ,同时 ,针刺“听宫”穴可以明显增强内耳热休克蛋白的表达水平 ,与其他 4组比较P <0 .0 1。针刺“后溪”和“肩贞”二穴对各项检测指标的影响作用不明显。结论 :并不是小肠经上的所有腧穴都与耳具有明显的相关性 ,只是局部穴位才具有这样的特点 ,体现了“腧穴所在 ,主治所在”的规律。

Study on the Specificity of Effects of Acupuncture of Different Acupoints in Reducing Gentamycin Induced Ototoxicity in Guinea-pigs

Objective: To observe the specific effect of acupuncture of acupoints of Small Intestine Meridian on the microstructure and expression of heat-shock protein (HSP 70) in the inner ear for analyzing the correlation between the Small Intestine Meridian and the ear. Methods: 60 Guinea-pigs were evenly randomized into control, model, “Tinggong”(SI 19),“Jianzhen” (SI 9) and “Houxi” (SI 3) groups. Apart from the control group, intramuscular injection of gentamycin (80 mg/kg/day, continuously for 20 days) was given to the animals of the other four groups to establish oto-intoxication model. Meanwhile, “Tinggong”(SI 19),“Jianzhen”(SI 9) and “Houxi”(SI 3) were punctured respectively and stimulated with even method, once (30 min) daily, and continuously for 20 days. At the end of experiments, the guinea-pig’s cochlear tissue was collected, fixed in 4% paraformaldehyde, sliced and stained by succino-dehydrogenase. The number of cochlear hair cells was calculated under light microscope, HSP 70 was assayed with immunohistochemical method and the microstructure of cochlear hair cells were observed under transmission electron microscope. Results:The deficit cochlear cells in model,“Jianzhen” and“Houxi”groups were considerably more than those of control group (2339.8±622.4, 1568.8±447.2 and 1679.4±423.5 vs 200.1±73.5, P<0.05～0.01), whereas, those of “Tinggong” group (829.6±190.7) were significantly fewer than those of model group (P<0.05), showing a protection effect of acupuncture on cochlear cells. The microstructural injury (arrangement disorder, mitochondrial swelling, cellular membrane and nucleus membrane shrinkage, core vacuole, etc.) of the cochlear hair cell in “Tinggong” group was obviously lighter than that of model group, showing a protection effect of acupuncture. The grey value of cochlear hair cells of “Tinggong” group was significantly higher than those of the rest 4 groups (P<0.01), suggesting that acupuncture of “Tinggong” could distinctly upregulate the expression of HSP 70 in the inner ear tissue. No significant changes were found in the deficit cell number, HSP 70 expression and cellular microstructure in “Jianzhen” and “Houxi” groups, indicating that the effect of acupuncture of “Tinggong” possessed a specific effect on Gentamycin induced oto-intoxication in Guinea-pigs. Conclusion: Not all the acupoints of the Small Intestine Meridian have a remarkable interrelationship with the ear, and only the local acupoint near the ear has the specific effect, suggesting that “where is an acupoint, where is the indication”.