| 人正常生育期子宫肌肉的鞘糖脂组成 |
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| 引用本文： | 邓会山,朱正美,夏泉,崔肇春. 人正常生育期子宫肌肉的鞘糖脂组成[J]. 大连医科大学学报, 1988, 0(4) |
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| 作者姓名： | 邓会山 朱正美 夏泉 崔肇春 |
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| 作者单位： | 大连医学院生化教研室，大连医学院生化教研室，大连医学院生化教研室，大连医学院生化教研室 |
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| 摘 要： | 从正常生育期人子宫肌肉组织分离、纯化了神经节苷脂(Gls)和中性鞘糖脂(N-GSL),测定了两类鞘糖脂的含量、对其组分进行 HPTLC 谱分析,并通过酶水解法对 Gls 主要成份作了鉴定。结果表明子宫肌肉组织的 Gls中 GD_3含量高达56. 6%,其次为 GM_3占16. 9%,GT_(1b)占9. 8%。此外,还有少量 GM_1、 GD_(1a)、与 GD_(1b)。N-GSL 主要组分为 CTH 和 CQH,各为30. 5%与35. 4%,次为 CDH 占16. 8%,CMH 与含多糖基(≥5个糖基)的组分各<10%。本样品每100克湿重神经节苷脂含量约3. 48mg(以唾液酸表示),N-GSL 为3. 71mg(以鞘氨醇表示)。
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| 关 键 词： | 子宫 鞘糖脂 GD_3 神经节苷脂 薄板层析 |
GLYCOSPHINGOLIPID OF HUMAN UTERUS |
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| Deng Huishan,et al.. GLYCOSPHINGOLIPID OF HUMAN UTERUS[J]. Journal of Dalian Medical University, 1988, 0(4) |
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| Authors: | Deng Huishan et al. |
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| Affiliation: | Deng Huishan,et al. Department of Biochemistry,Dalian Medical College |
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| Abstract: | Gangliosides(Gls)and neutral glycosphingolipids(NGSL)were isolated and purified from human myometrium by DEAE-Celluloseand Iatrobeads column chromatography.The contents of Gls and NGSLwere determined by spectrophotometry,and the compositions were ana-lyzed by HPTLC.The major Gls component GD_3,which was purifiedby salt gradient elution from ion exchange column,was identified byenzymatic hydrolysis.GD_3 content was found to be the highest(56. 6%of total sialie acid),while those of GM_3 and GT_(1b) were 16. 9% and9. 8%,respectively.Other Gls's were GM_1,GD_(1a) and GD_(1b),(about 5%each).The major components of NGSL were CTH and CQH,(30. 5%and 35. 4%),then CDH(16. 8%);CMH and polysaccharide containingcomponents were less than 10% each.The contents of total LBSA and NGSL were 3. 48mg and 3. 71mg per100 grams of wet weight tissus. |
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| Keywords: | Uterus giycosphingolipid GD_3 ganglioside thin-layer chromatograph |
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