牛蒡子总木脂素代谢化学及其动态研究

目的:研究牛蒡子总木脂素代谢化学及其动态。方法:采用高效液相色谱法,色谱条件为:色谱柱PhenomenexLunaC₁₈(4.6mm×150mm,5μm),流动相乙腈-0.05%H₃PO₄(36:64),流速0.6mL·min^-1,检测波长为210nm,柱温25℃。结果:口服后5min在血浆与脏器中即可检出;血浆中主要代谢产物为牛蒡子苷元;肝脏等脏器中主要代谢产物除牛蒡子苷元外,还有一未确定的代谢产物。结论:高效液相色谱受杂质干扰小,稳定性和重复性较好,适合牛蒡子木脂素代谢产物测定;木脂素在血浆与肝脏等脏器中的代谢有明显区别。

Dyuamical studies on metabolic chemistry of ligan from seeds of Arctium lappa

OBJECTIVE: To study the metabolic chemistry and pharmaco-dynamics characters of ligan from seeds of Arctium lappa. METHOD: HPLC method was used in the study. The analysis was carried out on C18 column. The mobile phase was CH3CN-0.05% H3PO4 (36:64) with flow-rate at 0.6 mL x min(-1) and wave-length of 210 nm. The column temperature was kept at 25 degrees C. RESULT: The results indicated that the ligan was detected in plasma and the main organs 5 min after po. The main metabolic production in plasma was arctigenin. In addition, arctigenin and an unknown product were found in metabolic production in the organs. CONCLUSION: The method was stable,simple and reproducible. It can be used to determine the metabolic product of the ligan. The metabolic chemistry of ligan in plasma was obviously different from that in the main organs.