| 消痹灵治疗骨性关节炎的机制探讨 |
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| 引用本文： | 魏玉玲,刘营杰,梁克玉. 消痹灵治疗骨性关节炎的机制探讨[J]. 中国组织工程研究与临床康复, 2001, 5(8): 64-65 |
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| 作者姓名： | 魏玉玲 刘营杰 梁克玉 |
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| 作者单位： | 湖北中医学院附属医院, |
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| 基金项目： | 湖北省教委资助课题,已鉴定(1999－015) |
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| 摘 要： | 目的探讨消痹灵对软骨细胞增殖的影响及治疗骨性关节炎的机理。方法取传一代的软骨细胞接种于2块96孔培养板和培养瓶中,培养24h,分8组实验以MTT法测细胞活性及增殖情况(光吸收值A);用考马斯亮蓝法测细胞总蛋白。结果2％～8％的消痹灵均能促进软骨细胞增殖及增强蛋白质的合成能力。结论消痹灵可对抗基质金属蛋白酶的降解,减轻对关节软骨细胞的损害。
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| 关 键 词： | 消痹灵 培养 /软骨细胞 塞唑蓝比色法 考马斯亮蓝比色法 |
| 文章编号： | 1007－5496(2001)04－0064－02 |
| 修稿时间： | 2001-01-02 |
The effect of chinesemedcine Xiao Bi Ling(XBL) on chondrocyte prliferation in vitro |
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| WEI Yu ling,LIU Ying je,LIANG Ke yu. The effect of chinesemedcine Xiao Bi Ling(XBL) on chondrocyte prliferation in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2001, 5(8): 64-65 |
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| Authors: | WEI Yu ling LIU Ying je LIANG Ke yu |
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| Affiliation: | WEI Yu-ling,LIU Ying-je,LIANG Ke-yu.The Affiliated Hospital of Hubei Col lege of Traditional Chinese Medicine,Wuhan 430061,China |
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| Abstract: | Objective In order to study the effect on chondrocyte proliferation and the mechanism of treating osteoarthritis(OA) by XBL,the experimant is carried out.Method The first－ subcuiture chondrocytes are implanted into two 96－ foramen culture plates and sixteen culture flasks.After 24 hours in cubation 8 groups are divided into as follows:No 1 group(control)5％ serum DMEM No,2～ 7 groups(XBL)5％ serum,0.5％ 、 1％ 、 2％ 、 4％ 、 8％ 、 16％ XBL－ DMEM are added respectively.NO 8 group no cell incubation.After culture of 24 and 48 hours ,two plates are detected for viablilty and proliferation respectively by MTT method(Light absorption value A),cell total protein is assessed by comassie Brilliatnt G－ 250 colormetric assay.Result 2％ ～ 8％ XBL can promote the proliferation and protein synthesis of chondrocyte.Conclusion XBL can protec degradation from Matrix metallo protzinase and decrease the impairment of chondrocyte. |
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| Keywords: | XiaoBiLing(XBL) culture/chondrocyte comassie Brilliatn MTT |
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