肝细胞癌患者外周血单个核细胞Foxp3 mRNA水平变化*

目的 检测初发现未治疗肝细胞癌（HCC）患者外周血单个核细胞（PBMCs）中 Foxp3 mRNA水平变化及其与血清AFP水平的关系。方法 在HCC患者60例和健康人20例，采用RT-PCR法检测PBMCs中Foxp3 mRNA水平，常规检测血清AFP水平。结果 HCC患者和健康对照组外周血Foxp3 mRNA水平分别为（0.976±0.073）和（0.772±0.083），差异有统计学意义（P<0.01）；经Pearson相关性分析显示，HCC患者外周血Foxp3 mRNA水平与AFP水平呈正相关（r＝0.226，P＝0.025）。结论 HCC患者PBMCs 中Foxp3水平明显升高，对肿瘤的发生发展可能具有一定的预测作用。

Foxp3 mRNA in peripheral blood mononuclear cells in patients with hepatocellular carcinoma

Objective To investigate Foxp3 mRNA levels in peripheral blood mononuclear cells （PBMCs） in patients with hepatocellular carcinoma（HCC） and explore its relationship with serum AFP levels. Methods 60 patients with HCC and 20 healthy persons were enrolled in this study. The Foxp3 mRNA in PBMCs was measured by RT-PCR and serum AFP was detected at the same time. Results There existed statistically significant difference in Foxp3 levels between patients with HCC and controls[（0.976±0.073） vs.（0.772±0.083），P<0.01]； Pearson correlation analysis showed positive correlation between Foxp3 levels in PBMCs and serum AFP levels in HCC patients（r＝0.226，P＝0.025）. Conclusion The Foxp3 levels in PBMCs in patients with HCC increase，which might help predict HCC development.