肝癌组织乙酰肝素酶的表达及其与微血管密度的关系

目的观察乙酰肝素酶（Hpa）在肝癌组织中的表达,并探讨其与微血管密度（MVD）的关系。方法采用RT-PCR法检测47例原发性肝癌组织、癌旁组织1（A组）和癌旁组织2（B组）Hpa mRNA水平;采用免疫组织化学法检测肝癌组织Hpa蛋白和CD34表达水平,并分析Hpa蛋白与肝癌临床病理特征的关系,以及Hpa mRNA水平与MVD值的相关性。结果在不同直径和分化程度、不同临床分期、是否转移复发、AFP水平和有无门脉癌栓等肿瘤组织,Hpa蛋白表达存在明显的差异（P<0.05）;肝癌组织Hpa mRNA水平（0.793±0.184）和MVD值（34.5±12.2）均明显高于A组和B组（P<0.05）,而A组Hpa mRNA水平（0.577±0.145）和MVD值（22.2±10.7）又明显高于B组（0.384±0.117）和（14.7±7.4）,P<0.05];各组Hpa mRNA水平与MVD值呈正相关（r=0.627,P<0.05）。结论Hpa在肝癌的生长、侵袭和血管生成中发挥着重要作用。

Expression of heparanase in liver cancer tissues and its relationship with microvascular density

Objective To explore the expression of heparanase（Hpa） in liver cancer tissues, and its relationship with the microvascular density（MVD）. Methods The Hpa mRNA levels in tumor, close-adjacent cancerous （group A） and peri-cancerous liver tissues （group B） from 47 patients with primary liver cancer were detected by RT-PCR. The expression of Hpa protein and CD34 in liver and cancer tissues were detected by immunohistochemistry. The correlation of Hpa mRNA to MVD in tissues with different clinical and pathological characteristics were analyzed. Results There were significant differences between Hpa levels in tumor tissues with different differentiation and clinical stages,with or without metastasis and recurrence,high or low AFP levels,with or without portal vein tumor emboli,and large or small tumor sizes （P<0.05）;The Hpa mRNA levels （0.793±0.184） and the MVD（34.5±12.2） in liver cancerous tissues were significantly higher than those in group A and group B （P<0.05）,while the Hpa mRNA levels and MVD in group A were significantly higher than those in group B （0.577±0.145） vs. （0.384±0.117）,and （22.2±10.7） vs. （14.7±7.4）,respectively,P<0.05];The Hpa mRNA level in each group was positively correlated with MVD（r=0.627,P<0.05）. Conclusion Hpa plays an important role in the growth,invasion and angiogenesis of hepatocellular carcinoma.