积雪草苷对小鼠脂多糖致痛觉增敏及对MPO、NO和TNF-α影响研究

 目的观察积雪草苷(aisaticoside,AS)对小鼠腹腔注射脂多糖(lipopolysaccharide,LPS)诱导痛觉增敏型疼痛的影响,并探讨AS对髓过氧化物酶(myeloperoxidase,MPO)、一氧化氮(nitrogen monoxidum,NO)和α-肿瘤坏死因子(α-tumour necrosis factor,TNF-α)的影响。方法随机将小鼠分为空白组、模型组、盐酸哌替啶组、AS5,15,45mg·kg^-1 6组,通过腹腔注射LPS100μg·kg^-1建立痛阈增敏型疼痛模型,采用醋酸扭体法、热板法观察AS的镇痛效应;通过相关试剂盒检测小鼠脑组织MPO和NO,ELISA法检测血浆TNF-α含量的变化。结果AS 5,15,45mg·kg^-1各组明显减少醋酸扭体次数,提高小鼠痛阈。降低脑组织MPO活性、NO含量和血浆TNF-α含量。结论AS具有明显镇痛效应,其镇痛效应可能与降低MPO、NO和TNF-α有关。

Studies on Analgesic Effect and Mechanism of Aisaticoside on Lipopolysaccharide Induced Hyperalgesia in Mice

OBJECTIVE To observe the analgesia effect of aisaticoside on hyperalgesia induced by intraperitoneal LPS in mice,and investigate the change of MPO,NO and TNF-α.METHODS Mice were divided randomly into normal group,model group,spasmedal group and AS 5,15,45 mg·kg^-1 group,hyperalgesia was induced by intraperitoneal administration of LPS 100 μg·kg^-1 in mice.The analgesia effect of AS was assessed by the hot-plate stimulation and the writhing test induced by acetic acid,the activity of MPO,NO in brain tissue was tested by kits;the content of TNF-α in plasma was tested by ELISA.RESULTS AS doses of 5,15,45 mg·kg^-1 significantly reduced the number of writhing,increased pain threshold,decreased the activity of MPO,NO and TNF-α.CONCLUSION AS have the effect of analgesia and its effect maybe related with inhibiting the MPO,NO and TNF-α.