Establishment and characterization of primary lung cancer cell lines from Chinese population |
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Authors: | Zheng Chao Sun Yi-hua Ye Xiao-lei Chen Hai-quan Ji Hong-bin |
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Institution: | Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, China. |
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Abstract: | Aim:To establish and characterize primary lung cancer cell lines from Chinese population.Methods:Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice.Results:Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations.Conclusion:These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response. |
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Keywords: | lung cancer primary cell lines epidermal growth factor receptor small cell lung carcinomas non-small cell lung carcinomas |
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