食管癌组织中CD44V6、VEGF、MMP-2的表达及其与临床病理特征的关系

目的探讨CD44拼接异构体(CD44V6)、血管内皮生长因子(VEGF)、基质金属蛋白酶(MMP)-2在食管癌组织中的表达及其与临床病理特征的关系.方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结(SP)法对60例食管癌患者的癌组织进行免疫组化研究.结果食管癌组织中CD44V6、VEGF、MMP-2的阳性表达率分别为68.33%、56.67%、70.00%;CD44V6阳性表达率与食管癌细胞分化程度无关(P＞0.05),与浸润深度、淋巴结转移均有关(均P＜0.05);VEGF与食管癌细胞浸润深度无关(P＞0.05),与分化程度、淋巴结转移均有关(均P＜0.05);MMP-2与食管癌细胞分化程度、浸润深度、淋巴结转移均有关(P＜0.05或0.01);20例患者(33.33%)3项指标均阳性,其中低分化、浸润程度深及有淋巴结转移者的阳性率分别高达58.82%(10/17)、47.06%(16/34)及56.52%(13/23).结论CD44V6、VEGF、MMP-2与食管癌的癌细胞分化程度、侵袭和转移密切相关,联合检测这3项指标对判断食管癌的生物学行为及预后具有重要意义.

The Expression of CD44V6,VEGF,MMP-2 in human esophageal carcinoma and its clinicopathological significance

Objective To observe the expression of CD44 splice variant V6 (CD44V6),vascular endothelial growth factor (VEGF),matrix metalloproteinase-2 (MMP-2) in human esophageal carcinoma,and its relation with clinicopathological features of the tumor. Methods Paraffin embedded sections from 60 samples of esophageal carcinomas were examined with the method of immunohistochemistry for CD44V6,VEGF and MMP-2 expression. Results The Positive rate of CD44V6,VEGF and MMP-2 expression was 68.33% (41/60),56.67%(34/60) and 70.00%(42/60) respectively. The expression of CD44V6 was closely related to invasion depth and lymph node metastasis of tumor,but not related to pathological grades. The expression of VEGF and MMP-2 was significantly related to pathological grades,invasion depth and lymph node metastasis of tumor. The expression of three proteins was 33.33%,which was significantly related to pathological grades,invasion depth and lymph node metastasis. Conclusion The expression of CD44V6,VEGF and MMP-2 are correlated with the development,invasion and metastasis of esophageal squamous cell carcinoma. Examination of expression of three proteins may be helpful to judge biological behavior and prognosis of esophageal carcinoma.