| 大鼠睾丸支持细胞雄激素结合蛋白mRNA在生精周期中的 … |
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| 引用本文： | Chen Y,Ye S,Huang Y,Zheng W. 大鼠睾丸支持细胞雄激素结合蛋白mRNA在生精周期中的 …[J]. 中国医学科学院学报, 2000, 22(3): 287-289 |
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| 作者姓名： | Chen Y Ye S Huang Y Zheng W |
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| 基金项目： | 国家自然科学基金! (39770 778) |
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| 摘 要： | 目的 研究大鼠睾丸支持细胞（ｓｅｒｔｏｌｉ ｃｅｌｌ,ＳＣ）雄激素结合蛋白（ＡＢＰ）ｍＲＮＡ水平与曲细精管上皮周期的相关关系。方法 以地高辛标记的大鼠ＡＢＰ ｃＤＮＡ探针在ＳＤ大鼠睾丸冰冻切片上进行原位杂交,应用激光密度扫描系统对阳性信号进行定量。结果 ＡＢＰ ｍＲＮＡ在１－６期维持低水平,７－８期迅速升高达到高峰,９～１１期骤然下降,但仍高于１～４期水平,７－１６期略有回升,随后迅速下降 至１～
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| 关 键 词： | 支持细胞 雄激素结合蛋白 睾丸 mRNA 生精周期 |
| 修稿时间： | 1998-12-26 |
Stage-dependent expression of androgen binding protein mRNA in sertoli cell of rat testis |
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| Chen Y,Ye S,Huang Y,Zheng W. Stage-dependent expression of androgen binding protein mRNA in sertoli cell of rat testis[J]. Acta Academiae Medicinae Sinicae, 2000, 22(3): 287-289 |
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| Authors: | Chen Y Ye S Huang Y Zheng W |
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| Affiliation: | Department of Histology and Embryology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China. |
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| Abstract: | OBJECTIVE: To investigate the variation of rat androgen binding protein (ABP) mRNA in sertoli cell of rat testis during spermatogenesis. METHODS: In situ hybridization was performed with digoxin-labeled rat ABP cDNA probe on frozen testis tissue sections from SD rats. The expression of ABP mRNA was quantified by using laser density scanning system. RESULTS: It was shown that ABP mRNA levels were low from stage I to stage VI, increased to the peak level at the stages VII-VIII, and then declined rapidly at stages IX-XI. Thereafter, ABP mRNA levels increased again at stages XII-XIV, and then decreased to the lowest level at stages I-VI. CONCLUSIONS: ABP mRNA level was stage-dependent during the spermatogenic cycle. |
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| Keywords: | sertoli cell androgen binding protein testis |
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