恒温越冬对蚂蟥生长及性腺发育的影响

为了探究恒温越冬对蚂蟥生长、性腺发育及内在品质的影响。将越冬期蚂蟥置于21℃的光照培养箱,以自然越冬蚂蟥为对照,越冬结束后测定蚂蟥的体质量、性腺质量及内在品质,石蜡切片观察蚂蟥精卵巢组织形态。结果表明,与自然越冬组相比,恒温越冬蚂蟥体质量是其2.35倍,雌雄性腺质量分别增加11.54%,48.33%,卵巢囊泡内卵黄期细胞和胶膜形成期细胞以及精巢囊腔内精原细胞和初级精母细胞均显著增加,抗凝血酶活性增加27.85%。综上,恒温能促进越冬蚂蟥生长、精卵细胞发育,提高抗凝血酶活性。

Effects of constant temperature on growth and gonad development of overwintering Whitmania pigra

The present study was conducted to explore the effect of constant temperature overwintering on the growth, gonadal development and internal quality of the Wh. pigra which were in overwintering period. Wh. pigra which in overwintering period were placed in light incubator at 21 ℃, and the Wh. pigra that overwintered under natural conditions were used as control. That the changes of growth performance, gonad index, internal quality of two groups of Wh. pigra were measured at the end of the overwintering. Simultaneously the tissue slice technique was used to observe the morphological structure of the spermary and ovary of the two groups. The results showed that the body weight of constant temperature overwintering Wh. pigra which were placed in light incubator at 21 ℃ was 2.35 times that of natural overwintering Wh. pigra which overwintered under natural conditions, and the weight of female gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 11.54% higher than that of Wh. pigra which overwintered under natural conditions, and the weight of male gonads of the Wh. pigra which were placed in light incubator at 21 ℃ was 48.33% higher than that of the Wh. pigra which overwintered under natural conditions. At the same time, that vitellogenesis cells and film forming cells which in ovarian vesicles were significantly higher than those of the Wh. pigra which overwintered under natural conditions, and that spermatogonia and primary spermatocytes which in seminal vesicle were significantly higher than those of Wh. pigra which overwintered under natural conditions. Most important of all, the anti-thrombin activity of Wh. pigra which were placed in light incubator at 21 ℃ increased by 27.85% compared with the Wh. pigra that overwintered under natural conditions. In conclusion, that constant temperature can promote the growth, the development of sperm and egg cells, and the increase of anti-thrombin activity of Wh. pigra which were in overwintering period.