肺炎支原体感染患者高迁移率族蛋白B1及其受体表达水平分析

目的探讨肺炎支原体感染患者高迁移率族蛋白B1(High mobility group protein B1,HMGB1)及其受体晚期糖基化终末产物受体(Receptor for advanced glycation end products,RAGE)表达变化及作用机制。方法选择2018年1月-2019年1月南阳市中心医院收治的肺炎支原体感染患者76例作为研究组,另选择同期健康体检者60名作为对照组。采用RT-qPCR实验检测HMGB1及其RAGE表达;采用罗氏E179全自动电化学发光分析仪及配套试剂盒测定降钙素原(Procalcitonin,PCT)含量;采用免疫比浊测定C-反应蛋白(C-reactive protein,CRP)含量。比较两组淋巴细胞绝对值、中性粒细胞绝对值和白细胞计数(white blood cell count,WBC)水平,血浆PCT和CRP水平变化,及HMGB1和RAGE表达变化。结果研究组淋巴细胞绝对值、中性粒细胞绝对值和WBC高于对照组,差异具有统计学意义(P<0.05)。研究组血浆PCT和CRP水平高于对照组,差异具有统计学意义(P<0.05)。研究组HMGB1和RAGE表达水平高于对照组,差异具有统计学意义(P<0.05)。结论肺炎支原体感染患者HMGB1和RAGE表达升高,认为可能参与了疾病的发生、发展过程,可用于早期鉴别肺炎支原体感染,值得临床借鉴。

Analysis of expression of high mobility group protein B1 and its receptor in patients with Mycoplasma pneumoniae infection

OBJECTIVE To investigate the expression of high mobility group protein B1(HMGB1)and its receptor advanced glycation end product receptor(RAGE)and its mechanism in patients with Mycoplasma pneumoniae infection.METHODS From January 2018 to January 2019,76 patients with M.pneumoniae infection in Nanyang central hospital were recruited as the observation group,and 60 healthy people in the same period were enrolled as the control group.The expression of HMGB1 and RAGE was detected by RT-qPCR;PCT content was determined by Roche E179 automatic electrochemiluminescence analyzer and kit,and CRP content was determined by immunoturbidimetry.The variation absolute value of lymphocyte and neutrophil,white blood cell count(WBC),plasma level of PCT and CRP,and expression of HMGB1 and RAGE were compared between the two groups.RESULTS The absolute value of lymphocyte,neutrophil and WBC in the observation group were higher than that in the control group(P<0.05).The plasma PCT and CRP levels in the observation group were significantly higher than that in the control group(P<0.05).The expression level of HMGB1 and RAGE in the observation group was higher than that in the control group(P<0.05).CONCLUSION The expression of HMGB1 and RAGE in patients with M.pneumoniae infection increased,which may be involved in the occurrence and development of the disease,and can be used for early identification of M.pneumoniae infection and worthy of clinical reference.