HPLC同时测定蓍草中6种主要成分的含量

建立同时测定蓍草中6种主要成分绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、墙草碱和新墙草碱B含量的色谱方法,用于指导蓍草的质量控制。以6种成分为对照品,采用Merck Purospher STAR RP-18色谱柱(4.6 mm×250 mm, 5μm),0.05%磷酸水(A)-乙腈(B)为流动相,梯度洗脱(0~7 min,12%~14%B;7~10 min,14%~17%B;10~25 min,17%~22%B;25~35 min,22%~35%B;35~51 min,35%~80%B;51~60 min,80%~90%B),流速1 mL·min^-1,检测波长254 nm,柱温30℃,进样量5μL。12批蓍草中绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、墙草碱和新墙草碱B质量分数分别为0.404%~2.116%,0.160%~0.892%,0.608%~1.464%,0.168%~0.868%,0.122%~1.234%,0.065%~0.312%。同时采用Chempattern软件对花期前和花期采收样品进行了聚类分析和主成分分析,不同采收期样品中主要成分含量具有明显的区别,采收时应注意采收期。该法可用于蓍草中6种主要成分的含量测定,为该药材质量标准的完善提供参考。

Simultaneous determination of 6 main components in Achilleae Herba by HPLC

This study aims to establish an HPLC method for the simultaneous determination of 6 main components,including chlorogenic acid,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid,pellitorine and neopellitorine B in Achilleae Herba.HPLC analysis was performed on a Merck Purospher STAR RP-18 endcapped(4.6 mm×250 mm,5μm),with a mobile phase consisting of 0.05%phosphoric acid solution(A)-acetonitrile(B)at a flow rate of 1 mL·min-1(0-7 min,12%-14%B;7-10 min,14%-17%B;10-25 min,17%-22%B;25-35 min,22%-35%B;35-51 min,35%-80%B;51-60 min,80%-90%B).The detection wavelength was 254 nm and the column temperature maintained at 30℃,and the injection volume was 5μL.The standard curves revealed a good linear relationship.The contents of 6 components were 0.404%-2.116%for chlorogenic acid,0.160%-0.892%for 3,4-dicaffeoylquinic acid,0.608%-1.464%for 3,5-dicaffeoylquinic acid,0.168%-0.868%for 4,5-dicaffeoylquinic acid,0.122%-1.234%for pellitorine,0.065%-0.312%for neopellitorine B,respectively.Both cluster and principal component analysis can distinguish the research data in anthesis and pre-anthesis by software Chempattern.There were obviously differences in the different harvest time.Therefore,attention should be paid to the harvesting time of the herb.The method can be used to determine the contents of six main components,and can provide reference for the improvement of quality standard of Achilleae Herba.