| 金银花配方颗粒质量快速检测体系研究 |
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| 引用本文： | 陈梓媛,王丽,蒋超,金艳,周骏辉,南铁贵,袁媛. 金银花配方颗粒质量快速检测体系研究[J]. 中国中药杂志, 2020, 0(5): 1070-1075 |
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| 作者姓名： | 陈梓媛 王丽 蒋超 金艳 周骏辉 南铁贵 袁媛 |
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| 作者单位： | 中国中医科学院中药资源中心 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(ZZ10-008);中央本级重大支减项目(2060302)。 |
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| 摘 要： | 中药配方颗粒在临床上的应用逐渐广泛,但目前缺乏快速简便的质量控制方法。该研究将位点特异性PCR技术和酶联免疫检测方法(ELISA)用于金银花配方颗粒质量的快速检测,即利用位点特异性PCR技术对金银花配方颗粒进行真伪鉴别,利用酶联免疫检测技术对金银花配方颗粒进行指标性成分的快速检测,从而为金银花配方颗粒质量快速检测标准建立奠定基础,也为其他中药配方颗粒质量标准的研究提供参考。
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| 关 键 词： | 金银花 配方颗粒 位点特异性PCR 酶联免疫检测方法 质量检测 |
Rapid quality detection system of Lonicerae Japonicae Flos formula granules |
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| CHEN Zi-yuan,WANG Li,JIANG Chao,JIN Yan,ZHOU Jun-hui,NAN Tie-gui,YUAN Yuan. Rapid quality detection system of Lonicerae Japonicae Flos formula granules[J]. China Journal of Chinese Materia Medica, 2020, 0(5): 1070-1075 |
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| Authors: | CHEN Zi-yuan WANG Li JIANG Chao JIN Yan ZHOU Jun-hui NAN Tie-gui YUAN Yuan |
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| Affiliation: | (National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China) |
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| Abstract: | The application of traditional Chinese medicine(TCM)formula granules in clinical practice is gradually extensive.However,TCM formula granules is still lacking rapid and simple quality control standards.In this study,allele-specific PCR and enzyme-linked immunoassay(ELISA)was used for rapid detection of the quality of Lonicerae Japonicae Flos formula granules.The authenticity of Lonicerae Japonicae Flos formula granules was identified by allele-specific PCR and index component was detected by ELISA.Thus,it lays a foundation for the establishment of rapid quality detection standard for Lonicerae Japonicae Flos formula granules,and also provides reference for other studies on the quality standard of traditional Chinese medicine formula granules. |
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| Keywords: | Lonicerae Japonicae Flos formula granule allele-specific PCR ELISA quality detection |
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