金银花分子生物学研究进展

分子生物学(molecular biology)是从分子水平阐明生命现象和本质的科学,其发展为传统生药学的研究提供了新的生物技术和方法。金银花作为常用大宗药材之一,国内外学者在深入研究传统方法的基础上,采用分子生物学手段对其展开真伪鉴别、品质评价和控制等方面的相关研究,并取得了一定成果。该文主要综述了近年来分子生物学技术方法在金银花鉴别、有效成分生物合成的分子机制以及胁迫条件下次生代谢产物积累的分子机制研究,并针对基于杂交技术的标记(RFLP)、基于PCR的分子标记(RAPD,AFLP,SSR,ISSR)和基于DNA序列分析的SNP及DNA条形码对金银花的多样性识别、诊断、鉴定等方面进行了详细的总结,同时提出可以采用多组学技术,构建系统生物学技术和平台,建立次生代谢产物生物合成的相关模型,从而更好地研究金银花活性成分生物合成的分子机制以及药用植物在环境胁迫下的相关代谢产物的合成和积累等生命活动规律并进行调控,为进一步推动金银花现代化及其他中药资源的开发利用提供支撑与参考。

Research progress in molecular biology of Lonicerae Japonicae Flos

Molecular biology is a new subject that clarifies the phenomena and nature of life at the molecular level.Its development provides new biotechnology and methods for the study of traditional pharmacognosy.The formation of molecular biology has brought the development of pharmacognosy into a new era of gene research.Lonicerae Japonicae Flos is a classical Chinese medicine.Many scholars of home and abroad have carried out relevant studies on its molecular biology on the basis of the in-depth study with traditional methods,and have achieved certain results.In order to provide references on the method,technical for promoting the modernization of Lonicerae Japonicae Flos,and the development,protection,and utilization of other traditional Chinese medicine resources.This article summarized the application status of molecular biology methods and techniques on the identification,biosynthesis of active constituents,and molecular mechanism of secondary metabolite under stress conditions of Lonicerae Japonicae Flos in recent years.In hybridization technology of tag(RFLP),molecular markers based on PCR(RAPD,AFLP,SSR and ISSR),based on DNA sequence analysis of SNP and DNA barcode for the variety identification,diagnosis,identification of Lonicerae Japonicae Flos,and so forth in detail.At the same time,it is proposed that multi-omics technology can be used to build systems biology technology and platforms,and establish related models of secondary metabolite biosynthesis,so as to deepen acknowledge the molecular mechanism of the active component biosynthesis of Lonicerae Japonicae Flos and the accumulation of metabolites,life activities of other medicinal plants under adverse environment,then to regulate them.