环状RNA-PCAC1促进胰腺导管腺癌侵袭转移的研究

目的探讨环状RNA(circRNA)-PCAC1在胰腺导管腺癌中的表达水平及其与患者临床病理特征、预后的关系。方法鉴定circRNA-PCAC1,并通过功能实验观察其对胰腺导管腺癌侵袭转移能力的影响。采用RT-PCR检测76例胰腺导管腺癌组织与配对癌旁组织的circRNA-PCAC1表达水平,Cox回归模型和Kaplan-Meier曲线法分析circRNA-PCAC1与患者临床病理特征和预后的关系。结果功能实验证实circRNA-PCAC1过表达显著促进胰腺导管腺癌侵袭转移能力。胰腺导管腺癌组织中circRNA-PCAC1的表达水平显著高于癌旁组织(P<0.01)。circRNA-PCAC1过表达与胰腺导管腺癌患者不良预后相关(P<0.05)。circRNA-PCAC1是胰腺导管腺癌患者总生存期(HR=1.733,95% CI:1.066~2.991,P=0.030)和无病生存期(HR=1.636,95% CI:1.090~2.811,P=0.042)的独立影响因素。结论 circRNA-PCAC1在胰腺导管腺癌中高表达并促进胰腺导管腺癌侵袭转移,提示其可能成为胰腺导管腺癌临床治疗的新靶点。

Circular RNA-PCAC1 promotes the metastasis of pancreatic ductal adenocarcinoma

ObjectiveTo determine the expression of circular RNA (circRNA) and evaluate the value of circRNA for clinicopathologic and prognostic of pancreatic ductal adenocarcinoma. MethodsThe construction of circRNA-PCAC1 was determined by RT-PCR using random primer or oligo-dT primer. The invasion of pancreatic ductal adenocarcinoma cells were measured by transwell assay in vitro. The expression of circRNA-PCAC1 in a cohort of 76 cases of pancreatic ductal adenocarcinoma tissues and paired normal adjacent tissues were detected by RT-PCR. The clinicopathologic information was collected and analyzed for the correlation with circRNA-PCAC1 expression by using Cox regression model and Kaplan-Meier curve analysis. ResultscircRNA-PCAC1 promoted pancreatic ductal adenocarcinoma cells invasion. The expression of circRNA-PCAC1 was up-regulated in pancreatic ductal adenocarcinoma compared with normal adjacent tissues (P<0.01). High expression of circRNA-PCAC1 was associated with poor prognosis in pancreatic ductal adenocarcinoma (P<0.05). circRNA-PCAC1 was an independent poor prognostic predictor related to overall survival (HR=1.733, 95% CI: 1.066-2.991, P=0.030) and disease free survival (HR=1.636, 95% CI: 1.090-2.811, P=0.042) rate of pancreatic patients. ConclusioncircRNA-PAPC1 is up-regulated in pancreatic ductal adenocarcinoma and promotes the invasion and metastasis of pancreatic ductal adenocarcinoma, supporting that circRNA-PCAC1 may be a potential therapeutic target for pancreatic ductal adenocarcinoma.