lncRNA相关因子对食管癌细胞增殖和侵袭的影响

目的研究lncRNA相关因子在食管癌细胞中对其增殖和侵袭生物学行为的调控,为食管癌的诊断和治疗提供依据。方法收集食管癌组织和癌旁组织样品各46份,分别提取RNA,采用RT-PCR检测MEG 3和linc02471,分析两者在食管癌组织和癌旁组织中表达的差异性。分别建立空载组和过表达组并进行细胞增殖试验,设计siRNA对MEG 3和linc02471进行抑制试验。采用Transwell小室对食管癌细胞中过载组、抑制组和空白组进行增殖活性和侵袭活性检测。结果 MEG 3和linc02471食道癌组织的相对表达量为(0.41±0.02)和(0.49±0.03),MEG 3和linc02471癌旁组织的相对表达量为(1.31±0.12)和(1.27±0.14)。MEG 3和linc02471在食道癌组织的表达水平低于对应的癌旁组织。抑制组MEG 3和linc02471相对表达量为(0.61±0.04)和(0.57±0.03),空白组MEG 3和linc02471相对表达量为(1.02±0.09)和(0.96±0.09),抑制组MEG 3和linc02471表达水平低于空白组。对基于MEG 3和linc02471设计的siRNA能抑制MEG 3和linc02471表达。MEG 3和linc02471的低表达时,食道癌细胞增殖活性上升。增殖活性和侵袭活性试验显示,MEG 3和linc02471过表达组食管癌细胞侵袭活性降低至原有活性的0.6~0.8倍,抑制组食管癌细胞侵袭活性提升至原有活性的1.5~2倍。结论 MEG 3和linc02471的过表达能够抑制食管癌细胞,可为肿瘤的诊断和治疗提供依据。

Study on lncRNA related factors and proliferation and invasion of esophageal cancer cells

Objective To study how lncRNA-related factors regulate the biological behavior(proliferation and invasion) of esophageal cancer cells in order to provide a basis for the diagnosis and treatment of esophageal cancer. Methods Forty-six samples of esophageal cancer and adjacent tissues were collected. RNA was extracted from esophageal cancer tissue and paracancerous tissue, and RT-PCR was used to detect the differential expression of MEG 3 and linc02471 in esophageal cancer tissue and paracancerous tissue. A cell proliferation test was performed on a blank group and an overexpression group, and an MEG 3 and linc02471 inhibition test was designed using siRNA. Transwell cells were used to detect the proliferation and invasion of esophageal cancer cells in the overexpression group, inhibition group, and blank group. Results The relative level of MEG 3 expression in esophageal cancer was 0.41 ± 0.02, and the relative level of linc02471 expression was 0.49 ± 0.03. The relative level of MEG 3 expression in adjacent tissues was 1.31 ± 0.12 and the relative level of linc02471 expression was 1.27 ± 0.14. The level of MEG 3 and linc02471 expression in esophageal cancer was significantly lower than that in adjacent tissues. The relative level of MEG 3 expression in the inhibition group was 0.61 ± 0.04, and the relative level of linc02471 expression was 0.57 ± 0.03. The relative level of MEG 3 expression in the blank group was 1.02 ± 0.09, and the relative level of linc02471 expression was 0.96 ± 0.09. The siRNA designed for MEG 3 and linc02471 effectively inhibited the expression of MEG 3 and linc02471. When the level of MEG 3 and linc02471 expression was low, the proliferative activity of esophageal cancer cells increased. The results of proliferation and invasive activity indicated that the invasive activity of esophageal cancer cells in the group overexpressing MEG 3 and linc02471 decreased to 0.6-0.8 times the original level of activity. The invasive activity of esophageal cancer cells in the inhibition group increased to 1.5-2 times the original level of activity. Conclusion Overexpression of MEG 3 and linc02471 inhibited esophageal cancer cells. Further study will provide a basis for the diagnosis and treatment of tumors.