人体髋臼前后柱骨硬度分布特征研究

目的研究人体髋臼前柱和后柱的硬度分布特征，探索骨硬度分布的科研价值及临床应用前景。 方法本研究所用两男一女3具标本均由河北医科大学解剖教研室提供，选取3对髋臼前后柱样本，取出全部髋骨后剔除软组织，运用微型台锯将髋骨分割为髋臼前柱和后柱，后用高精慢速锯将髋臼前后柱切割成若干厚3 mm的骨组织切片，固定在载玻片后用砂纸打磨。使用维氏硬度仪测试骨组织切片不同解剖区域皮质骨和松质骨的硬度值。本研究采用50 g力加载50 s、维持12 s标准操作方法测定，组间数据比较采用配对t检验。 结果本研究在人体髋臼前柱和髋臼后柱随机切取骨片，代表了髋臼前后柱纵向的不同位置，测量共计450个有效值，3个样本髋臼前柱总体硬度范围在18.9～33.8 HV，均值(25.2±3.1)HV；髋臼后柱硬度范围在23.1～39.1 HV，均值(31.9±3.8) HV。男性捐献者髋臼前、后柱总体硬度范围分别为18.8～33.8 HV及23.1～39.1 HV；女性捐献者髋臼前、后柱总体硬度范围分别为19.4～33.8 HV及24.6～38.9 HV，所有标本中后柱平均骨硬度值均大于前柱，且差异有统计学意义(t＝7.502、4.724、5.487，P<0.01)，研究数据表明男性和女性髋臼前柱与后柱均存在硬度分布差异。 结论人体髋臼前后柱骨硬度值个体间存在差异，但均为后柱骨硬度大于前柱。骨硬度值的研究可为髋臼骨折内植物的置入位置、置钉长度及方向等提供参考，同时为研发符合人体梯度弹性模量的内植物及骨盆髋臼假体提供可靠的数据支撑。

Research on human bone microhardness distribution characteristics of acetabulum anterior and posterior columns

Objective To analysis the hardness distribution characteristics of the anterior and posterior column of the acetabulum,and to explore the value of bone hardness distribution in scientific research and clinical application.Methods The metatarsal bones in this study were collected from three healthy donors(two males,one female,Hebei Medical University).Three pairs of anterior and posterior acetabular column were selected,all the soft tissue was removed.Bone specimens with a thickness of 3 mm were taken from anterior and posterior acetabular column with a low-speed saw.The microhardness of the bone tissue was measured using a Vickers microhardness tester after polish.The indentation load and dwell time was set to 50 g and 12 s in this study.The paired t test was used for data comparison between groups.Results Bone specimens,which were randomly cut from the anterior acetabular column and the posterior acetabular column in human body,representing different longitudinal positions of the anterior and posterior acetabular columns.Totally,450 valid values at different bones and anatomic sites were involved in our result.The total hardness of the acetabular anterior column ranged from 18.9 to 33.8 HV with a mean of(25.2±3.1)HV and that of the acetabular posterior column ranged from 23.1 to 39.1 HV with a mean value of(31.9±3.8)HV.Bone hardness of acetabular anterior column and posterior column of male donor ranged from 18.8 to 33.8 HV and 23.1 to 39.1 HV respectively.For the female donor,the total hardness of acetabular anterior column and posterior column were 19.4-33.8 HV and 24.6-38.9 HV,the average bone hardness of posterior column of the acetabulum was larger than that of the anterior column,and the difference was statistically significant(t=7.502、4.724、5.487,all P<0.01).The data showed that there was a difference in hardness distribution between the anterior and posterior column of the acetabulum in both men and women.Conclusions There are individual differences for the hardness of acetabular,and the hardness of posterior column is higher than that of anterior column.This study can provide a reference for the position of implants,length and direction of screw,as well as provide reliable data support for the development of implants and acetabular prostheses in line with the gradient elastic modulus of human body.