薰鲁香化学成分研究

综合运用各种现代色谱分离技术从薰鲁香甲醇提取物中分离得到9个萜类成分,采用NMR,MS并与相关文献对照鉴定其结构为:3β,8α,13-trihydroxypolypoda-14-methoxy-14-methyl-17,21-diene(1),4-hydroxymyrtenal(2),3-methyl-6-(prop-1-en-2-yl)cyclohex-3-ene-1,2-diol(3),2-oxo-Δ^3-4,5,5-trimethylcyclopentynyl acidic acid(4),(1S,2R,3R,5R)-6,6-dimethyl-4-methylidenebicyclo3.1.1]-heptane-2,3-diol(5),(4R)-1-methyl-4-(1-hydroxyisopropyl)cyclohexene-6-one(6),6,6-dimethyl-4-hydroxy3.1.1]hept-2-ene-2-carboxylic acid(7),6,6-dimethyl3.1.1]hept-2-ene-2-carboxylic acid(8),6,6-dimethyl-4-oxobicyclo3.1.1]hept-2-ene-2-carboxylic acid(9)。其中化合物1为未见文献报道的新化合物,2~9为首次从薰鲁香中分离得到。体外细胞毒活性筛选结果显示,化合物1,6,7对人肺癌细胞A549有明显的细胞毒作用,IC^50分别为20.4,25.1,22.5μmoL·L^-1。

Study on chemical constituents from Mastic(Pistacia lentiscus)

Phytochemical investigation on the methanolic extract of Mastic by using various chromatographic techniques led to the isolation of 9 compounds.Based on the analysis of spectroscopic data(NMR and MS)and/or comparisons with the data reported in the literature,their structures were elucidated as 3β,8α,13-trihydroxypolypoda-14-methoxy-14-methyl-17,21-diene(1),4-hydroxymyrtenal(2),3-methyl-6-(prop-1-en-2-yl)cyclohex-3-ene-1,2-diol(3),2-oxo-Δ^3-4,5,5-trimethylcyclopentynyl acidic acid(4),(1S,2R,3R,5R)-6,6-dimethyl-4-methylidenebicyclo3.1.1]-heptane-2,3-diol(5),(4R)-1-methyl-4-(1-hydroxyisopropyl)cyclohexene-6-one(6),6,6-dimethyl-4-hydroxy3.1.1]hept-2-ene-2-carboxylic acid(7),6,6-dimethyl3.1.1]hept-2-ene-2-carboxylic acid(8),6,6-dimethyl-4-oxobicyclo3.1.1]hept-2-ene-2-carboxylic acid(9).Compound 1 is a new compound and 2-9 were isolated from this species for the first time.In vitro cytotoxicity assay results indicated that compounds 1,6 and 7 showed significant inhibitory effects against human lung cancer cell line A549 with IC50 values of 20.4,25.1 and 22.5μmoL·L^-1.