肾癌组织与正常肾组织中热休克蛋白gp96-肽复合物体外免疫效应比较

目的:研究人肾癌组织中提取、纯化的热休克蛋白(HSP)gp96 肽复合物的体外免疫效应。方法:分别从12例肾癌患者肿瘤组织标本及5例肾外伤患者切除的正常肾组织中提取、纯化 HSPgp96 肽复合物。应用酶联免疫斑点(ELISPOT)法检测肾癌患者肿瘤组织,正常肾组织中提取的HSPgp96 肽复合物、单纯多肽刺激人外周血单核细胞(PMBC)产生γ 干扰素效应T细胞频数。结果:肿瘤组中 HSPgp96 肽复合物产生γ 干扰素效应 T细胞频数最高每孔(287.6±22.3)个],肿瘤组中HSPgp96 肽复合物,单纯多肽产生γ 干扰素效应 T细胞频数明显高于正常肾组织组(P<0.01)。两组中HSPgp96 肽复合物与单纯多肽产生γ 干扰素效应T细胞频数存在明显差异(P<0.05)。结论:肾肿瘤组织中提取的HSPgp96 肽复合物具有明显最强的体外免疫效应。

Comparison of Immune Effect of HSPgp96-peptide Complex Derived and Purified from Human Renal Tumor and Normal Renal Tissues

Objective: To investigate the immune effect in vitro of heat shock protein (HSP) gp96-peptide complex derived and purified from human renal carcinoma. Methods: HSPgp96-peptide was derived, purified and evaluated from 12 human renal tumor tissues and 5 normal renal tissues. Enzyme-linked immunospot assay (Elispot) was used to detect the frequency of interferon(IFN)-γ +T cell produced by peripheral blood mononuclear cells(PMBCs), which stimulated by HSPgp96-peptide complex and polypeptide derived from human carcinoma and normal renal tissues. Results: HSPgp96-peptide complex from renal carcinoma produced maximal frequency of IFN-γ +T cells (287.6±22.3 per hole). The HSPgp96-peptide complex and polypeptide from renal carcinoma produced more frequency of IFN-γ +T cells than that from normal renal tissues (P<0.01). The frequency of IFN-γ +T cells stimulated by HSPgp96-peptide complex and polypeptide derived from two different renal tissues had significant difference (P<0.05). Conclusion: HSPgp96-peptide complex derived from human renal carcinoma has the strongest immune effect in vitro.