正交试验法优化松花粉中酚类化合物超声提取工艺

目的：优化松花粉中酚类化合物的超声提取工艺。方法：采用正交设计试验对松花粉中酚类化合物超声提取过程中乙醇浓度、超声时间、超声温度等因素进行优化。结果：超声提取松花粉酚类化合物的最佳工艺条件为：乙醇浓度40%,超声提取时间30分钟,超声提取温度50℃,在此条件下,松花粉酚类化合物提取率可达4.2 mg/g。结论：采用超声辅助提取松花粉酚类化合物,时间短、得率高,是一种高效、快捷的方法。

Orthogonal Test Optimizing Ultrasonic Extraction of Phenolic Compound from Chinese Pine Pollen

Objective： To optimize ultrasonic extraction technology of phenolic compound from Chinese pine pollen. Method： factors such as ethanol concentration, extraction time, temperature and others, which influenced ultra- sonic extraction of phenolic compound, were optimized through orthogonal test. Result： The best condition was deter- mined as： 40% of ethanol concentration, 30min of extraction time and extraction temperature 50℃, under the condi- tion, maximal extraction yield of phenolic compound reached 4.2mg/g. Conclusion： Ultrasonic extraction technology is an effective and rapid method for extracting phenols from Chinese Pine Pollen.