妊娠期高血压疾病胎盘组织中CASR和EGFR的相关研究

目的探讨在妊娠期高血压疾病患者胎盘组织中细胞外钙受体（CASR）和表皮生长因子受体（EGFR）的表达情况及其相互关系。方法通过免疫组织化学方法检测妊娠期高血压疾病患者64例（妊娠期高血压组21例,子痫前期轻度组23例,重度组20例）及健康足月孕妇20例（对照组）胎盘组织中CASR和EGFR蛋白的表达情况。结果 （1）妊娠期高血压疾病患者胎盘组织中CASR在妊娠期高血压组表达水平为59.0532±8.039,子痫前期轻度组患者中为64.3623±3.7278,两组比较,差异有统计学意义（P〈0.0001）;CASR在子痫前期重度组患者中表达为112.2831±6.2060,与妊娠期高血压组比较,差异有统计学意义（P〈0.0001）,与子痫前期轻度组比较,差异有统计学意义（P〈0.0001）。CASR的蛋白在妊娠期高血压组患者胎盘组织中表达水平为59.0532±8.039,对照组为54.8585±4.3035,两组比较,差异无显著性（t=0.08,P=0.7759）。（2）妊娠期高血压疾病患者胎盘组织中表皮生长因子受体（EGFR）在妊娠期高血压组表达水平为56.174±3.1020,子痫前期轻度组患者中为78.6844±2.6713,两组比较,差异有统计学意义（t=18.73,P=0.0001）;EGFR在子痫前期重度组患者中表达为94.2090±6.8352,与子痫前期轻度组比较,差异有统计学意义（t=11.37,P〈0.0001）。（3）胎盘组织中CASR和表皮生长因子受体（EGFR）表达量呈正相关关系（r=0.352,P〈0.05）。结论妊娠期高血压疾病患者胎盘组织中CASR的蛋白表达升高和EGFR激活及过度增高,可能在妊娠期高血压疾病发生发展中起重要作用。

Related study on the role of CASR and EGFR in placental of hypertensive disorder complicating pregnancy

0bjective: To investigate the Related Study on the Role of CASR and EGFR in Hypertensive Disorder Complicating Pregnancy.Methods: Immunohistochemistry used to investigate the expression of CASR and EGFR proteins in placental from gestational hypertension,mild preeclampsia,severe preeclampsia and normal term pregnant women.Results:(1)Expression of CASR proteins level in placenta of gestational hypertension was significantly lower than that in mild preeclampsia(P<0.0001).Expression of CASR proteins in placenta of mild preeclampsia was significantly lower than that severe preeclampsia,P<0.0001.There was no significantly difference in CASR proteins level between gestational hypertension and normal term pregnancy(q=0.08,P=0.7759).(2)Expression of EGFR proteins level in placenta of mild preeclampsia higher than that gestational hypertension(P<0.0001).Expression of EGFR proteins in placenta of severe preeclampsiawas significantly higher than that mild preeclampsia,P<0.0001.(3)The proteins level of CASR in placental acute atherosclerosis was positively correlated with EGFR.Conclusion:It suggested that abnormal proteins expressions of CASR and activation of EGFR may play important roles in the placental of hypertensive disorder complicating pregnancy.