| 抗人PCIA1单克隆抗体的制备及其生物特征的研究 |
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| 引用本文： | 阚兵,严飞,杨金亮,邓洪新,王瑞,魏于全. 抗人PCIA1单克隆抗体的制备及其生物特征的研究[J]. 华西医学, 2006, 21(1): 84-85 |
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| 作者姓名： | 阚兵 严飞 杨金亮 邓洪新 王瑞 魏于全 |
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| 作者单位： | 生物治疗国家重点实验室,肿瘤生物治疗研究室,四川大学华西医院,四川成都,610041 |
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| 摘 要： | 目的:筛选制备抗人PCIA1的单克隆抗体,鉴定其生物学特征。方法:采用原核表达并纯化的PCIA1蛋白作为抗原,采用杂交瘤技术制备抗人PCIA1单克隆抗体的杂交瘤,用ELISA进行筛选和鉴定其亚类,并用细胞扒片免疫化学方法检测其反应性。结果:获得了32株分泌抗人PCIA1的McAb杂交瘤细胞,McAb-1A4的染色体呈双亲特点,该McAb与多种人肿瘤细胞株和正常肝细胞株有反应。结论:McAb-1A4可用于PCIA1的细胞内定位研究。
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| 关 键 词： | PCIA1 单克隆抗体 杂交瘤 |
| 文章编号： | 1002-0179(2006)01-0084-02 |
| 收稿时间： | 2005-05-13 |
| 修稿时间： | 2005-05-13 |
Production of Monoclonal Antibody Against Human PCIA1 and Study on Its Characteristics |
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| KAN Bing, YAN Fei, YANG Jin-liang,et al.. Production of Monoclonal Antibody Against Human PCIA1 and Study on Its Characteristics[J]. West China Medical Journal, 2006, 21(1): 84-85 |
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| Authors: | KAN Bing YAN Fei YANG Jin-liang et al. |
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| Affiliation: | State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective:To prepare monoclonal antibody(McAb) against human PCIA1 protein and study its biological activity.Methods:As an antigen recombinant PCIA1 protein was used for immunizing Balb/c mice.McAbs against PCIA1 protein was prepared by hybridoma technique,ELISA was used to select hybridomas and identify its immunoglobin subclass.Attach cell model also was used to identify where PCIA1 existed in cells.Results:Thirty-two hybridomas which secreted McAb against PCIA1 were obtained.The chromosomes of McAb-1A4 showed the characteristics of their parents.The immunoglobulin subclass of McAb-1A4 was IgG1.This McAb presented reactions to all tumor cell lines and hepatocellular normal cell line adopted.Conclusion:McAb-1A4 is able to be used to localize the site of PCIA1 in cells. |
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| Keywords: | PCIA1 monoclonal antibody hybridoma |
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