| 高效液相色谱法测定人血浆中硝苯地平的浓度 |
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| 引用本文： | 黄一玲,边文彦,崔华东,宋珍,李一石. 高效液相色谱法测定人血浆中硝苯地平的浓度[J]. 中国临床药学杂志, 1999, 0(Z1) |
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| 作者姓名： | 黄一玲 边文彦 崔华东 宋珍 李一石 |
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| 作者单位： | 中国协和医科大学阜外心血管病医院临床药理研究室 北京100037 |
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| 摘 要： | 目的:建立高效液相色谱法测定人血浆中硝苯地平的浓度.方法:分析柱为Micro Pah C 18分析柱.流动相为甲醇:水:冰乙酸:正丁胺(77:23:0.02:O.01).检测波长为235nm.采取乙醚二次萃取法提取血浆中的硝苯地平及内标安定.结果:测定硝苯地平的日内及日间精密度的RSD均小于10%.血药浓度在6.16～197.20 ng/ml范围内线性关系良好(r=0.9994).最低检测限为2ng/ml.结论:本方法适用于硝苯地平的药物动力学研究.
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| 关 键 词： | 高效液相色谱 硝苯地平 血药浓度 |
High performance liquid chromatographic assay for the nifedipine in human plasma |
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| Huang Yiling,Bian Wenyan,Cui Huadong,et al. High performance liquid chromatographic assay for the nifedipine in human plasma[J]. Chinese Journal of Clinical Pharmacy, 1999, 0(Z1) |
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| Authors: | Huang Yiling Bian Wenyan Cui Huadong et al |
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| Affiliation: | Huang Yiling,Bian Wenyan,Cui Huadong,et al Department of Clinical Pharmacology,Fuwai Hospital,CAMS and PUMC,Beijing 100037 |
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| Abstract: | AIM: To establish high performance liquid chromatography for the determina-tion of nifedipine in human plasma. METHODS: Separation of nifedipine and internal standard,diazepam, was obtained using Micro Pak C 18 analysis column. The detection was done at 235 nm. The mobile phase consisted of methanol-water-acetic acid-buthylamine(77 : 23 : 0.02 : 0. 0l, V/V). They were extracted from plasma with diethyl ether. RESULTS: The relative standard deviation (RSD)of within-day and day-to-day were less than 10%. Calibration was linear from 6. 16 to 197. 20 ng/ml(r=0. 999 4). The limit of detection was 2 ng/ml. CONCLUSION: This method has been demonstrated to be suitable for pharma-cokinetic study. |
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| Keywords: | high performance liquid chromatography nifedipine plasma concentration |
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