PYY3-36与饮食诱导肥胖抵抗的关系

目的研究高脂饮食诱导肥胖(DIO)及肥胖抵抗(DIOR)大鼠血浆PYY336含量及回结肠PYYmRNA的表达情况,探讨PYY336与高脂饮食诱导肥胖抵抗的关系。方法36只雌性SD大鼠,按体重随机分为高脂实验组(n=27)和基础对照组(n=9),分别给予高脂饲料和基础饲料喂养13周。据13周末体重将高脂饲料组再分为饮食诱导肥胖和饮食诱导肥胖抵抗组,比较各组大鼠能量摄入水平、血浆PYY336含量及回结肠PYYmRNA表达水平的差异。结果DIOR大鼠热能摄入量显著低于DIO大鼠(P<0.01),与对照组大鼠相比无显著性差异(P>0.05);DIOR大鼠血浆PYY336含量及回结肠PYYmRNA表达水平显著高于DIO和对照组大鼠(P<0.01);DIO与对照组相比,除回肠PYYmRNA表达水平升高外,其余指标均无显著性差异(P>0.05)。结论高脂饮食条件下,SD大鼠表现为明显的肥胖易感性差异,这种差异可能与PYY336的食欲调节密切相关,血浆PYY336含量和回结肠PYYmRNA表达水平的升高可能是导致DIOR大鼠热能摄入下降的内在机制之一。

Relation between peptide YY 3-36 and dietary induced obesity resistant rats

Objective To study the plasma concentration of peptide YY3-36(PYY3-36) and the expression levels of PYY mRNA of ileum and colon in dietary induced obesity (DIO) and dietary induced obesity resistant (DIO-R) rats and the relation between PYY3-36 and DIO-R rats. Methods Thirty-six female SD rats were randomly divided into high-fat diet group (n=27) and chow fed control group (n=9), after 13 weeks of either a high-fat diet or chew fed diet, the high-fat diet group was subdivided into DIO and DIO-R group according to the final body weight. Weight gain, caloric intake, the concentration of PYY3-36 and the expression levels of PYY mRNA were measured and compared.Results The total caloric intake of DIO-R rats was lower than DIO rats (P<0.01),while no significant difference was found between DIO-R and control rats (P>0.05). The concentration of PYY3-36 and the expressions of PYY mRNA of ileum and colon in DIO-R rats were higher significantly than that of the DIO and control rats (P<0.01), while no significant difference was found between DIO and control group (P>0.05), except that PYY mRNA of ileum was advanced in DIO group (P<0.01). Conclusion On the High-fat diet, SD rats showed different susceptibility to obese and energy intake, increased levels of PYY3-36 and PYY mRNA might be related to dietary induced obesity resistant.