广西家鼠型肾综合征出血热病毒的发现和鉴定

目的 从广西各地捕获的鼠肺标本检测汉坦病毒并鉴定其型别.方法 结合广西鼠疫监测采集啮齿类宿主动物,用ELISA法检测鼠肺标本,对汉坦病毒抗原阳性标本通过RT-PCR法扩增部分M片段上的核苷酸序列并测序,将扩增片段的核苷酸序列与已知病毒序列进行比对并分型.结果 在306份鼠肺标本中检测到1株SEO型的出血热病毒,阳性标本为广西沿海地区钦州市捕获的褐家鼠.结论 在广西沿海地区检测到SEO型汉坦病毒,其流行病学意义有待进一步研究.

Identification and analysis of an SEO strain of hemorrhagic fever with renal syndrome hantavirus from Guangxi

Objective To identify hantavirus from lung specimens of rats captured in Guangxi. Methods Rats were collected from various areas in Guangxi in combination with plague surveillance and rat lung specimens were examined by ELISA for hantavirus antigen,and M segment of positive specimen was partially amplified with RT-PCR and sequenced. Phylogenetic analysis was conducted for genotyping. Results From a total of 306 rat lung specimens,a strain of SEO hantavirus was detected from lung specimen of a Norvegicus that was from coastal area of Guangxi Qinzhou city. Conclusions For the first time SEO hantanviras was detected in the coastal area of Guangxi Qinzhou city,its epidemiological significance needs further study.