自酸蚀牙本质粘接剂对人牙髓成纤维细胞的毒性作用

目的比较和评价自酸蚀粘接剂XenoⅢ（XO）和Adper Prompt（AP）以及全酸蚀粘接剂Single bond2（SB）三者的细胞毒性大小。方法将3种牙本质粘接剂XO、AP和SB涂布于直径为5.0 mm、厚度为0.5 mm的牙本质圆片的两面，置于DMEM培养液中获得材料的浸提液，然后将培养液稀释成100.0%、50.0%、25.0%和12.5%四种体积分数。选用组织块法体外原代培养人牙髓成纤维细胞，并将不同体积分数的材料浸提液与第5代人牙髓成纤维细胞共同培养，通过MTT法评价材料24、72、120 h的细胞毒性。结果牙本质粘接系统XO、AP和SB在体外对人牙髓成纤维细胞均有一定程度的细胞毒性，两种自酸蚀粘接剂XO和AP的细胞毒性明显低于全酸蚀粘接剂SB，其差异有统计学意义（P<0.05）。结论自酸蚀牙本质粘接剂XO和AP的细胞毒性小于全酸蚀牙本质粘接剂SB。

Effect of the self-etching adhesives system on human pulp fibroblast

OBJECTIVE: To compare and evaluate the biocompatibility of three kinds of dentin bonding agents Xeno III (XO), Adper Prompt (AP), Single bond2 (SB) through cell culture in vitro. METHODS: Three kinds of dentin bonding agents (XO, AP, SB) were applied on the surface of the dental slices which were 5.0 mm in diameter and 0.5 mm in depth. By immersing the slices into the DMEM culture medium, the maceration extracts were obtained. Normal dental pulps of teenagers were collected and human pulp fibroblast was cultured using tissue explant method. The fifth generation pulp cells were exposed to culture medium containing different concentrations of maceration extracts (100.0%, 50.0%, 25.0%, 12.5%) for 24, 72, 120 h. At last, MTT method was used to evaluate the cytotoxicity of the dentin bonding agents on human pulp fibroblast. RESULTS: The results showed that all three kinds of dentin bonding systems had cytotoxicity to human pulp fibroblast in different degree in vitro. The cytotoxicity of XO and AP was less than SB. The difference was statistically significant (P<0.05). CONCLUSION: The results of cell culture in vitro indicated that total-etching adhesives system has more irritation to pulp than self-etching adhesives system.